Background. Strategies aimed at antiretroviral therapy (ART)-free remission will target individuals with a limited viral reservoir. We investigated factors associated with low reservoir measured as total human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood mononuclear cells (PBMCs) in perinatal infection (PaHIV).Methods. Children from 7 European centers in the Early Treated Perinatally HIV Infected Individuals: Improving Children's Actual Life (EPIICAL) consortium who commenced ART aged <2 years, and remained suppressed (viral load [VL] <50 copies/mL) for >5 years were included. Total HIV-1 DNA was measured by quantitative polymerase chain reaction per million PBMCs. Factors associated with total HIV-1 DNA were analyzed using generalized additive models. Age, VL at ART initiation, and baseline CD4% effects were tested including smoothing splines to test nonlinear association.Results. Forty PaHIV, 27 (67.5%) female 21 (52.5%) Black/Black African, had total HIV-1 DNA measured; median 12 (IQR, 7.3-15.4) years after ART initiation. Eleven had total HIV-1 DNA <10 copies/10(6) PBMCs. HIV-1 DNA levels were positively associated with age and VL at ART initiation, baseline CD4%, and Western blot antibody score. Age at ART initiation presented a linear association (coefficient = 0.10 +/- 0.001, P <= .001), the effect of VL (coefficient = 0.35 +/- 0.1, P <= .001) noticeable >6 logs. The effect of CD4% (coefficient = 0.03 +/- 0.01, P <= .049) was not maintained >40%.Conclusions. In this PaHIV cohort, reduced total HIV-1 DNA levels were associated with younger age and lower VL at ART initiation. The impact of early-infant treatment on reservoir size persists after a decade of suppressive therapy.
Foster, C., Dominguez-Rodriguez, S., Tagarro, A., Gkouleli, T., Heaney, J., Watters, S., et al. (2021). The CARMA Study: Early Infant Antiretroviral Therapy - Timing Impacts on Total HIV-1 DNA Quantitation 12 Years Later. JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY, 10(3), 295-301 [10.1093/jpids/piaa071].
|Tipologia:||Articolo su rivista|
|Citazione:||Foster, C., Dominguez-Rodriguez, S., Tagarro, A., Gkouleli, T., Heaney, J., Watters, S., et al. (2021). The CARMA Study: Early Infant Antiretroviral Therapy - Timing Impacts on Total HIV-1 DNA Quantitation 12 Years Later. JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY, 10(3), 295-301 [10.1093/jpids/piaa071].|
|Settore Scientifico Disciplinare:||Settore MED/38|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/jpids/piaa071|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2021|
|Titolo:||The CARMA Study: Early Infant Antiretroviral Therapy - Timing Impacts on Total HIV-1 DNA Quantitation 12 Years Later|
|Autori:||Foster, C; Dominguez-Rodriguez, S; Tagarro, A; Gkouleli, T; Heaney, J; Watters, S; Bamford, A; Fidler, K; Navarro, M; De Rossi, A; Palma, P; Nastouli, E; Rossi, P; Giaquinto, C; Rojo, P|
|Appare nelle tipologie:||01 - Articolo su rivista|