Several thermal areas, also used for leisure purposes, may represent suitable habitats for free-living amoebae (FLAs), but few studies have been carried out in search for these organisms. The aim of this study was to assess the presence and distribution of FLAs by culture detection and molecular identification, over a one year-round sampling of two sites in Central Italy. Two geothermal springs (Site A and Site B) were investigated for a total of 36 water samples. Four sets of primers were used to amplify FLA DNA from all cultures positive for amoebic growth at both 37 °C and 45 °C. Overall, 33 (91.6%) water samples produced PCR amplification. Eleven taxa were identified. The array of identified species varied over the sampling period, and differed between the two hot springs, Site A harbouring 11 taxa compared to 5 of site B. However, both sites were characterized by the most common species Vermamoeba vermiformis and Naegleria australiensis. Acanthamoeba genotypes T4 and T15 were found at low frequency. Differences in the composition between the two sites could reflect environmental changes in biotic and chemical/physical parameters. From a public health perspective, the detection of potentially pathogenic amoebae could unveil a potential risk for humans.

Berrilli, F., Di Cave, D., Novelletto, A., Montalbano Di Filippo, M. (2021). PCR-based identification of thermotolerant free-living amoebae in Italian hot springs. EUROPEAN JOURNAL OF PROTISTOLOGY, 80, 125812 [10.1016/j.ejop.2021.125812].

PCR-based identification of thermotolerant free-living amoebae in Italian hot springs

Berrilli, Federica
;
Di Cave, David;Novelletto, Andrea;
2021

Abstract

Several thermal areas, also used for leisure purposes, may represent suitable habitats for free-living amoebae (FLAs), but few studies have been carried out in search for these organisms. The aim of this study was to assess the presence and distribution of FLAs by culture detection and molecular identification, over a one year-round sampling of two sites in Central Italy. Two geothermal springs (Site A and Site B) were investigated for a total of 36 water samples. Four sets of primers were used to amplify FLA DNA from all cultures positive for amoebic growth at both 37 °C and 45 °C. Overall, 33 (91.6%) water samples produced PCR amplification. Eleven taxa were identified. The array of identified species varied over the sampling period, and differed between the two hot springs, Site A harbouring 11 taxa compared to 5 of site B. However, both sites were characterized by the most common species Vermamoeba vermiformis and Naegleria australiensis. Acanthamoeba genotypes T4 and T15 were found at low frequency. Differences in the composition between the two sites could reflect environmental changes in biotic and chemical/physical parameters. From a public health perspective, the detection of potentially pathogenic amoebae could unveil a potential risk for humans.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06
English
Free-living amoebae; Potentially pathogenic species; Molecular identification; Community composition; Geothermal springs; Italy
Berrilli, F., Di Cave, D., Novelletto, A., Montalbano Di Filippo, M. (2021). PCR-based identification of thermotolerant free-living amoebae in Italian hot springs. EUROPEAN JOURNAL OF PROTISTOLOGY, 80, 125812 [10.1016/j.ejop.2021.125812].
Berrilli, F; Di Cave, D; Novelletto, A; Montalbano Di Filippo, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/276116
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