Type 2 transglutaminase (TG2) is a multifunctional protein involved in various biological processes playing a key regulatory role in cell homeostasis such as cell death and autophagy. New evidence is emerging that support an important role of autophagy in regulating normal hematopoiesis. Prompted by these findings, in this study we investigated in vivo involvement of TG2 in mouse hematopoiesis under normal or nutrient deprivation conditions. We found that the number and rate of differentiation of bone marrow hematopoietic stem cell was decreased in the TG2 knockout mice. We present evidence showing that these effects on hematopoietic system are very likely due to the TG2-dependent impairment of autophagy. In fact, stimulation of autophagy by starvation is able to rescue the block of the differentiation of stem cells progenitors in the TG2 KO mice. It was also shown that the RhoA/ERK1/2 pathway, known to be essential for regulation of the bone marrow progenitor cells homeostasis, was significantly impaired in the absence of TG2. Hence, this study expanded our knowledge about TG2 discovering a role of this enzyme in regulation of hematopoiesis.

Oliverio, S., Beltran, J., Occhigrossi, L., Bordoni, V., Agrati, C., D'Eletto, M., et al. (2020). Transglutaminase Type 2 is Involved in the Hematopoietic Stem Cells Homeostasis. BIOCHEMISTRY, 85(10), 1159-1168 [10.1134/S0006297920100041].

Transglutaminase Type 2 is Involved in the Hematopoietic Stem Cells Homeostasis

Oliverio S.;D'Eletto M.;Rossin F.;Piacentini M.
2020-01-01

Abstract

Type 2 transglutaminase (TG2) is a multifunctional protein involved in various biological processes playing a key regulatory role in cell homeostasis such as cell death and autophagy. New evidence is emerging that support an important role of autophagy in regulating normal hematopoiesis. Prompted by these findings, in this study we investigated in vivo involvement of TG2 in mouse hematopoiesis under normal or nutrient deprivation conditions. We found that the number and rate of differentiation of bone marrow hematopoietic stem cell was decreased in the TG2 knockout mice. We present evidence showing that these effects on hematopoietic system are very likely due to the TG2-dependent impairment of autophagy. In fact, stimulation of autophagy by starvation is able to rescue the block of the differentiation of stem cells progenitors in the TG2 KO mice. It was also shown that the RhoA/ERK1/2 pathway, known to be essential for regulation of the bone marrow progenitor cells homeostasis, was significantly impaired in the absence of TG2. Hence, this study expanded our knowledge about TG2 discovering a role of this enzyme in regulation of hematopoiesis.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/06 - ANATOMIA COMPARATA E CITOLOGIA
English
Con Impact Factor ISI
TG2
hematopoietic stem cells
autophagy
Animals
Cell Differentiation
Cells, Cultured
Female
GTP-Binding Proteins
Hematopoiesis
MAP Kinase Signaling System
Mice
Mice, Inbred C57BL
Mice, Knockout
Transglutaminases
Autophagy
Hematopoietic Stem Cells
Oliverio, S., Beltran, J., Occhigrossi, L., Bordoni, V., Agrati, C., D'Eletto, M., et al. (2020). Transglutaminase Type 2 is Involved in the Hematopoietic Stem Cells Homeostasis. BIOCHEMISTRY, 85(10), 1159-1168 [10.1134/S0006297920100041].
Oliverio, S; Beltran, Jso; Occhigrossi, L; Bordoni, V; Agrati, C; D'Eletto, M; Rossin, F; Borelli, P; Amarante-Mendes, Gp; Demidov, O; Barlev, Na; Pia...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/272083
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