Amphiphilic poly( vinyl alcohol) randomly grafted with hydrophobic methacryloyl groups can form micelle-like particles by intra and interpolymeric association. Self-aggregation behaviour of the hydrophobically-modified polymer was investigated. The hydrophobized nanoparticles assist carbonic anhydrase B (CAB) refolding in a manner similar to the mechanism of molecular chaperones, namely by catching and releasing the protein. Irreversible CAB thermal denaturation is prevented by nanoparticle complexation and recovery of almost 100% of enzymatic activity is triggered by the ability of beta-cyclodextrin to interact with the hydrophobic moieties. Structural and functional properties of micelle-like particles were discussed and interpreted in view of the stability and architecture of hydrophobic nanodomains.
Cavalieri, F., Chiessi, E., Paradossi, G. (2007). Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol). SOFT MATTER, 3(6), 718-724 [10.1039/b618779j].
Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol)
CAVALIERI, FRANCESCA;
2007-01-01
Abstract
Amphiphilic poly( vinyl alcohol) randomly grafted with hydrophobic methacryloyl groups can form micelle-like particles by intra and interpolymeric association. Self-aggregation behaviour of the hydrophobically-modified polymer was investigated. The hydrophobized nanoparticles assist carbonic anhydrase B (CAB) refolding in a manner similar to the mechanism of molecular chaperones, namely by catching and releasing the protein. Irreversible CAB thermal denaturation is prevented by nanoparticle complexation and recovery of almost 100% of enzymatic activity is triggered by the ability of beta-cyclodextrin to interact with the hydrophobic moieties. Structural and functional properties of micelle-like particles were discussed and interpreted in view of the stability and architecture of hydrophobic nanodomains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
