The satellite cells (SCs) associated with muscle fibres play a key role in postnatal growth and regeneration of skeletal muscle. Commonly used methods of isolation and in vitro culture of SCs lead to the mixture of their subpopulations that exist within muscle. To solve this problem, we used the well established technique, the hanging drop system, to culture SCs in a three-dimensional environment and thus, to monitor them in their original niche.
Archacka, K., Pozzobon, M., Repele, A., Rossi, C.a., Campanella, M., De Coppi, P. (2014). Culturing muscle fibres in hanging drop: a novel approach to solve an old problem. BIOLOGY OF THE CELL, 106(2), 72-82 [10.1111/boc.201300028].
Culturing muscle fibres in hanging drop: a novel approach to solve an old problem
Campanella, Michelangelo
;
2014-02-01
Abstract
The satellite cells (SCs) associated with muscle fibres play a key role in postnatal growth and regeneration of skeletal muscle. Commonly used methods of isolation and in vitro culture of SCs lead to the mixture of their subpopulations that exist within muscle. To solve this problem, we used the well established technique, the hanging drop system, to culture SCs in a three-dimensional environment and thus, to monitor them in their original niche.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
