Tumor-infiltrating lymphocytes play an essential role in improving clinical outcome of neuroblastoma (NB) patients, but their relationship with other tumor-infiltrating immune cells in the T cell-inflamed tumors remains poorly investigated. Here we show that dendritic cells (DCs) and natural killer (NK) cells are positively correlated with T-cell infiltration in human NB, both at transcriptional and protein levels, and associate with a favorable prognosis. Multiplex imaging displays DC/NK/T cell conjugates in the tumor microenvironment of low-risk NB. Remarkably, this connection is further strengthened by the identification of gene signatures related to DCs and NK cells able to predict survival of NB patients and strongly correlate with the expression of PD-1 and PD-L1. In summary, our findings unveil a key prognostic role of DCs and NK cells and indicate their related gene signatures as promising tools for the identification of clinical biomarkers to better define risk stratification and survival of NB patients.

Melaiu, O., Chierici, M., Lucarini, V., Jurman, G., Conti, L., De Vito, R., et al. (2020). Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma. NATURE COMMUNICATIONS, 11(1) [10.1038/s41467-020-19781-y].

Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma

Melaiu O;Cifaldi L;
2020-11-01

Abstract

Tumor-infiltrating lymphocytes play an essential role in improving clinical outcome of neuroblastoma (NB) patients, but their relationship with other tumor-infiltrating immune cells in the T cell-inflamed tumors remains poorly investigated. Here we show that dendritic cells (DCs) and natural killer (NK) cells are positively correlated with T-cell infiltration in human NB, both at transcriptional and protein levels, and associate with a favorable prognosis. Multiplex imaging displays DC/NK/T cell conjugates in the tumor microenvironment of low-risk NB. Remarkably, this connection is further strengthened by the identification of gene signatures related to DCs and NK cells able to predict survival of NB patients and strongly correlate with the expression of PD-1 and PD-L1. In summary, our findings unveil a key prognostic role of DCs and NK cells and indicate their related gene signatures as promising tools for the identification of clinical biomarkers to better define risk stratification and survival of NB patients.
nov-2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - PATOLOGIA GENERALE
English
Con Impact Factor ISI
Adolescent
Adult
B7-H1 Antigen
Child
Child, Preschool
Cohort Studies
Datasets as Topic
Dendritic Cells
Disease-Free Survival
Female
Humans
Infant
Killer Cells, Natural
Lymphocytes, Tumor-Infiltrating
Male
Middle Aged
Neuroblastoma
Prognosis
Programmed Cell Death 1 Receptor
RNA-Seq
Sensitivity and Specificity
Survival Rate
T-Lymphocytes
Transcriptome
Tumor Microenvironment
Young Adult
Melaiu, O., Chierici, M., Lucarini, V., Jurman, G., Conti, L., De Vito, R., et al. (2020). Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma. NATURE COMMUNICATIONS, 11(1) [10.1038/s41467-020-19781-y].
Melaiu, O; Chierici, M; Lucarini, V; Jurman, G; Conti, L; De Vito, R; Boldrini, R; Cifaldi, L; Castellano, A; Furlanello, C; Barnaba, V; Locatelli, F; Fruci, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/264018
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