DNA-templated synthesis takes advantage of the programmability of DNA-DNA interactions to accelerate chemical reactions under diluted conditions upon sequence-specific hybridization. While this strategy has proven advantageous for a variety of applications, including sensing and drug discovery, it has been so far limited to the use of nucleic acids as templating elements. Here, we report the rational design of DNA templated synthesis controlled by specific IgG antibodies. Our approach is based on the co-localization of reactants induced by the bivalent binding of a specific IgG antibody to two antigen-conjugated DNA templating strands that triggers a chemical reaction that would be otherwise too slow under diluted conditions. This strategy is versatile, orthogonal and adaptable to different IgG antibodies and can be employed to achieve the targeted synthesis of clinically-relevant molecules in the presence of specific IgG biomarker antibodies.

Baranda Pellejero, L., Mahdifar, M., Ercolani, G., Watson, J., Brown, T., Ricci, F. (2020). Using antibodies to control DNA-templated chemical reactions. NATURE COMMUNICATIONS, 11(1) [10.1038/s41467-020-20024-3].

Using antibodies to control DNA-templated chemical reactions

Ercolani, Gianfranco;Ricci, Francesco
2020-01-01

Abstract

DNA-templated synthesis takes advantage of the programmability of DNA-DNA interactions to accelerate chemical reactions under diluted conditions upon sequence-specific hybridization. While this strategy has proven advantageous for a variety of applications, including sensing and drug discovery, it has been so far limited to the use of nucleic acids as templating elements. Here, we report the rational design of DNA templated synthesis controlled by specific IgG antibodies. Our approach is based on the co-localization of reactants induced by the bivalent binding of a specific IgG antibody to two antigen-conjugated DNA templating strands that triggers a chemical reaction that would be otherwise too slow under diluted conditions. This strategy is versatile, orthogonal and adaptable to different IgG antibodies and can be employed to achieve the targeted synthesis of clinically-relevant molecules in the presence of specific IgG biomarker antibodies.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01
Settore CHIM/06
English
algorithms; antibodies; DNA; DNA replication; kinetics; models, chemical; models, genetic; models, molecular; nucleic acid conformation; nucleic acid hybridization; nucleic acids; templates, genetic
https://www.nature.com/articles/s41467-020-20024-3
Baranda Pellejero, L., Mahdifar, M., Ercolani, G., Watson, J., Brown, T., Ricci, F. (2020). Using antibodies to control DNA-templated chemical reactions. NATURE COMMUNICATIONS, 11(1) [10.1038/s41467-020-20024-3].
Baranda Pellejero, L; Mahdifar, M; Ercolani, G; Watson, J; Brown, T; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/262354
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