The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains ceCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses. (c) 2006 Elsevier Inc. All rights reserved.

Ferrari, F., Mercaldo, V., Piccoli, G., Sala, C., Cannata, S., Achsel, T., et al. (2007). The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines. MOLECULAR AND CELLULAR NEUROSCIENCES, 34(3), 343-354 [10.1016/j.mcn.2006.11.015].

The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines

CANNATA, STEFANO;BAGNI, CLAUDIA
2007

Abstract

The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains ceCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses. (c) 2006 Elsevier Inc. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
eng
Con Impact Factor ISI
LONG-TERM POTENTIATION; CAMKII MESSENGER-RNA; SINGLE-STRANDED-DNA; MOUSE MODEL; PUR-ALPHA; BC1 RNA; HIPPOCAMPAL-NEURONS; NERVOUS-SYSTEM; SYNAPTIC PLASTICITY; ACTIN CYTOSKELETON
Ferrari, F., Mercaldo, V., Piccoli, G., Sala, C., Cannata, S., Achsel, T., et al. (2007). The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines. MOLECULAR AND CELLULAR NEUROSCIENCES, 34(3), 343-354 [10.1016/j.mcn.2006.11.015].
Ferrari, F; Mercaldo, V; Piccoli, G; Sala, C; Cannata, S; Achsel, T; Bagni, C
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/26166
Citazioni
  • ???jsp.display-item.citation.pmc??? 59
  • Scopus 86
  • ???jsp.display-item.citation.isi??? 83
social impact