Interaction between thyroid hormones (THs) and the immune system is reported in the literature. Thyroid hormone (THs), thyroxine, T4, but also T3 act nongenomically through mechanisms that involve a plasma membrane receptor αvβ3 integrin, a co-receptor for Insulin-like growth factor-1 (IGF-1). Previous data from our laboratory show a cross-talk between THs and IGF-1 because THs inihibit the IGF-1-stimulated glucose uptake and cell proliferation in L-6 myoblasts and the effects are mediated by integrin αvβ3. IGF-1 also behaves as a chemokine, being an important factor for tissue regeneration after damage. In the present study, using THP-1 human leukemic monocytes, expressing αvβ3 integrin in their cell membrane, we focused on the cross-talk between THs and either IGF-1 or monocyte chemoattractant protein-1 (MCP-1) studying cell migration and proliferation stimulated by the two chemokines, and the role of αvβ3 integrin, using inhibitors of αvβ3 integrin and downstream pathways. Our results show that IGF-1 is a potent chemoattractant in THP-1 monocytes stimulating cell migration and thyroid hormone inhibits the effect through αvβ3 integrin. Thyroid hormone also inhibits IGF-1-stimulated cell proliferation through αvβ3 integrin, an example of a cross-talk between genomic and nongenomic effects. We also studied the effects of Thyroid hormone on cell migration and proliferation induced by MCP-1, together with the pathways involved, by a pharmacological approach and docking simulation. Our findings show a different downstream signalling for IGF-1 and MCP-1 in THP-1 monocytes mediated by the plasma membrane receptor of THs, integrin αvβ3.

Candelotti, E., De Luca, R., Megna, R., Maiolo, M., De Vito, P., Percario, Z., et al. (2021). Thyroid hormones inhibit cell migration and proliferation activated by IGF-1 and MCP-1 in THP-1 monocytes through integrin αvβ3 by different mechanisms. FRONTIERS IN ENDOCRINOLOGY.

Thyroid hormones inhibit cell migration and proliferation activated by IGF-1 and MCP-1 in THP-1 monocytes through integrin αvβ3 by different mechanisms.

De Vito P.
Membro del Collaboration Group
;
Pedersen J. Z.
Writing – Review & Editing
;
2021-01-01

Abstract

Interaction between thyroid hormones (THs) and the immune system is reported in the literature. Thyroid hormone (THs), thyroxine, T4, but also T3 act nongenomically through mechanisms that involve a plasma membrane receptor αvβ3 integrin, a co-receptor for Insulin-like growth factor-1 (IGF-1). Previous data from our laboratory show a cross-talk between THs and IGF-1 because THs inihibit the IGF-1-stimulated glucose uptake and cell proliferation in L-6 myoblasts and the effects are mediated by integrin αvβ3. IGF-1 also behaves as a chemokine, being an important factor for tissue regeneration after damage. In the present study, using THP-1 human leukemic monocytes, expressing αvβ3 integrin in their cell membrane, we focused on the cross-talk between THs and either IGF-1 or monocyte chemoattractant protein-1 (MCP-1) studying cell migration and proliferation stimulated by the two chemokines, and the role of αvβ3 integrin, using inhibitors of αvβ3 integrin and downstream pathways. Our results show that IGF-1 is a potent chemoattractant in THP-1 monocytes stimulating cell migration and thyroid hormone inhibits the effect through αvβ3 integrin. Thyroid hormone also inhibits IGF-1-stimulated cell proliferation through αvβ3 integrin, an example of a cross-talk between genomic and nongenomic effects. We also studied the effects of Thyroid hormone on cell migration and proliferation induced by MCP-1, together with the pathways involved, by a pharmacological approach and docking simulation. Our findings show a different downstream signalling for IGF-1 and MCP-1 in THP-1 monocytes mediated by the plasma membrane receptor of THs, integrin αvβ3.
2021
In corso di stampa
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Thyroid hormones; Nongenomic effects; Thyroid hormones and IGF-1 Cross-talk; Integrin αvβ3; MCP-1 induced proliferation;
Candelotti, E., De Luca, R., Megna, R., Maiolo, M., De Vito, P., Percario, Z., et al. (2021). Thyroid hormones inhibit cell migration and proliferation activated by IGF-1 and MCP-1 in THP-1 monocytes through integrin αvβ3 by different mechanisms. FRONTIERS IN ENDOCRINOLOGY.
Candelotti, E; De Luca, R; Megna, R; Maiolo, M; De Vito, P; Percario, Z; Borgatti, M; Gambari, R; Davis, Pj; Lin, Hy; Polticelli, F; Persichini, T; Co...espandi
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/261245
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact