Cholangiopathies, such as primary sclerosing cholangitis, biliary atresia, and cholangiocarcinoma have limited experimental models. Not only cholangiocytes but also other hepatic cells including hepatic stellate cells and macrophages are involved in the pathophysiology of cholangiopathies, and these hepatic cells orchestrate the coordinated response against diseased conditions. Classic two-dimensional monolayer cell cultures do not resemble intercellular cell-to-cell interaction and communication; however, three-dimensional cell culture systems, such as organoids and spheroids, can mimic cellular interaction and architecture between hepatic cells. Previous studies have demonstrated the generation of hepatic or biliary organoids/spheroids using various cell sources including pluripotent stem cells, hepatic progenitor cells, primary cells from liver biopsies, and immortalized cell lines. Gene manipulation, such as transfection and transduction can be performed in organoids, and established organoids have functional characteristics which can be suitable for drug screening. This review summarizes current methodologies for organoid/spheroid formation and a potential for three-dimensional hepatic cell cultures as novel in vitro models of cholangiopathies.

Sato, K., Zhang, W., Safarikia, S., Isidan, A., Chen, A.m., Li, P., et al. (2020). Organoids and spheroids as novel models for studying cholestatic liver injury and cholangiocarcinoma. HEPATOLOGY [10.1002/hep.31653].

Organoids and spheroids as novel models for studying cholestatic liver injury and cholangiocarcinoma

Baiocchi, Leonardo;
2020-11-22

Abstract

Cholangiopathies, such as primary sclerosing cholangitis, biliary atresia, and cholangiocarcinoma have limited experimental models. Not only cholangiocytes but also other hepatic cells including hepatic stellate cells and macrophages are involved in the pathophysiology of cholangiopathies, and these hepatic cells orchestrate the coordinated response against diseased conditions. Classic two-dimensional monolayer cell cultures do not resemble intercellular cell-to-cell interaction and communication; however, three-dimensional cell culture systems, such as organoids and spheroids, can mimic cellular interaction and architecture between hepatic cells. Previous studies have demonstrated the generation of hepatic or biliary organoids/spheroids using various cell sources including pluripotent stem cells, hepatic progenitor cells, primary cells from liver biopsies, and immortalized cell lines. Gene manipulation, such as transfection and transduction can be performed in organoids, and established organoids have functional characteristics which can be suitable for drug screening. This review summarizes current methodologies for organoid/spheroid formation and a potential for three-dimensional hepatic cell cultures as novel in vitro models of cholangiopathies.
22-nov-2020
In corso di stampa
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/12 - GASTROENTEROLOGIA
English
bile ducts
cholangiocarcinoma
cholangiocytes
organoids
spheroids
Sato, K., Zhang, W., Safarikia, S., Isidan, A., Chen, A.m., Li, P., et al. (2020). Organoids and spheroids as novel models for studying cholestatic liver injury and cholangiocarcinoma. HEPATOLOGY [10.1002/hep.31653].
Sato, K; Zhang, W; Safarikia, S; Isidan, A; Chen, Am; Li, P; Francis, H; Kennedy, L; Baiocchi, L; Alvaro, D; Glaser, S; Ekser, B; Alpini, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/259877
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