Three isoinhibitors of bovine pancreatic trypsin inhibitor (BPTI) have been identified and isolated from bovine pituitary gland. The results of the purification process by affinity chromatography on immobilized trypsin, the electrophoretic mobility in non-denaturing conditions, the antiproteolytic activity and the immunochemical reactions indicate that these inhibitors correspond to those previously isolated from bovine spleen and lung. In addition, immunohistochemical experiments show that the isoinhibitors and BPTI are exclusively localized in the mast cells, and not in the endocrine cells, of the pars intermedia and posterior lobe (neurohypophysis) of the pituitary gland. The physiological implications of these findings are discussed.

Fiorucci, L., De Renzis, G., Businaro, R., Fumagalli, L., Fioretti, E., Giardina, B., et al. (1989). Identification and immunohistochemical localization of various bovine pancreatic trypsin inhibitor-isoforms in bovine pituitary gland. HISTOCHEMICAL JOURNAL, 21(12), 721-730 [10.1007/BF01002838].

Identification and immunohistochemical localization of various bovine pancreatic trypsin inhibitor-isoforms in bovine pituitary gland

Fiorucci, L;
1989-12-01

Abstract

Three isoinhibitors of bovine pancreatic trypsin inhibitor (BPTI) have been identified and isolated from bovine pituitary gland. The results of the purification process by affinity chromatography on immobilized trypsin, the electrophoretic mobility in non-denaturing conditions, the antiproteolytic activity and the immunochemical reactions indicate that these inhibitors correspond to those previously isolated from bovine spleen and lung. In addition, immunohistochemical experiments show that the isoinhibitors and BPTI are exclusively localized in the mast cells, and not in the endocrine cells, of the pars intermedia and posterior lobe (neurohypophysis) of the pituitary gland. The physiological implications of these findings are discussed.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10
English
Animals
Cattle
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Immunoglobulins
Immunohistochemistry
Isomerism
Lung
Male
Mast Cells
Pituitary Gland
Pituitary Gland, Posterior
Trypsin Inhibitor, Kazal Pancreatic
Trypsin Inhibitors
Fiorucci, L., De Renzis, G., Businaro, R., Fumagalli, L., Fioretti, E., Giardina, B., et al. (1989). Identification and immunohistochemical localization of various bovine pancreatic trypsin inhibitor-isoforms in bovine pituitary gland. HISTOCHEMICAL JOURNAL, 21(12), 721-730 [10.1007/BF01002838].
Fiorucci, L; De Renzis, G; Businaro, R; Fumagalli, L; Fioretti, E; Giardina, B; Ascoli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/258266
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