Proliferation and migration of gonocytes, the precursors of spermatogonial stem cells, to the germline niche in the basal membrane of the seminiferous tubules, are two crucial events that take place between postnatal d 0.5 (P0.5) and P5.0 in the mouse and involve a selection of the cells that are committed to the germline stem cells lineage. Here we show that from embryonic d 18.0 (E18) and up to P5, the gonocytes express platelet-derived growth factor (PDGF) receptor beta-subtype (PDGFR-beta) and that during the same time period, the Sertoli cells express PDGF-B and PDGF-D, both ligands for PDGFR-beta. Inhibition of the PDGFR-beta tyrosine kinase activity during the first five postnatal days provokes a profound reduction of gonocyte number through inhibition of their proliferation and induction of apoptosis. Moreover, we found that PDGFR-beta ligands are chemotactic for gonocytes. These data suggest that PDGFR-beta activation has the remarkable capability to drive the selection, survival, and migration of the gonocytes from the center of the seminiferous tubules to the testicular germline niche on the basal membrane.

Basciani, S., De Luca, G., DOLCI IANNINI, S., Brama, M., Arizzi, M., Mariani, S., et al. (2008). Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes. ENDOCRINOLOGY, 149(12), 6226-6235 [10.1210/en.2008-0349].

Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes

DOLCI IANNINI, SUSANNA;
2008-12-01

Abstract

Proliferation and migration of gonocytes, the precursors of spermatogonial stem cells, to the germline niche in the basal membrane of the seminiferous tubules, are two crucial events that take place between postnatal d 0.5 (P0.5) and P5.0 in the mouse and involve a selection of the cells that are committed to the germline stem cells lineage. Here we show that from embryonic d 18.0 (E18) and up to P5, the gonocytes express platelet-derived growth factor (PDGF) receptor beta-subtype (PDGFR-beta) and that during the same time period, the Sertoli cells express PDGF-B and PDGF-D, both ligands for PDGFR-beta. Inhibition of the PDGFR-beta tyrosine kinase activity during the first five postnatal days provokes a profound reduction of gonocyte number through inhibition of their proliferation and induction of apoptosis. Moreover, we found that PDGFR-beta ligands are chemotactic for gonocytes. These data suggest that PDGFR-beta activation has the remarkable capability to drive the selection, survival, and migration of the gonocytes from the center of the seminiferous tubules to the testicular germline niche on the basal membrane.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/16
English
Con Impact Factor ISI
Cell Movement; Platelet-Derived Growth Factor; Animals; Receptor, Platelet-Derived Growth Factor beta; Apoptosis; Testis; Spermatozoa; Mice; Reverse Transcriptase Polymerase Chain Reaction; Cell Proliferation; Proto-Oncogene Proteins c-sis; Mice, Knockout; Animals, Newborn; Mice, Inbred Strains; Piperazines; Blotting, Western; Sertoli Cells; Pyrimidines; Sperm Count; Phosphorylation; Mice, Inbred C57BL; Immunohistochemistry; Female; Male
Basciani, S., De Luca, G., DOLCI IANNINI, S., Brama, M., Arizzi, M., Mariani, S., et al. (2008). Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes. ENDOCRINOLOGY, 149(12), 6226-6235 [10.1210/en.2008-0349].
Basciani, S; De Luca, G; DOLCI IANNINI, S; Brama, M; Arizzi, M; Mariani, S; Rosano, G; Spera, G; Gnessi, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/25747
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