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EnglishRTN1-C protein is a membrane protein localized in the ER and expressed in the nervous system. Its biological role is still unclear, although interactions of the N-terminal region of RTN1-C with proteins involved in vesicle trafficking have been observed, but the role of the C-terminal region of this family protein remains to be investigated. By a homology analysis of the amino acid sequence, we identified in the C-terminal region of RTN1-C a unique consensus sequence characteristic of H4 histone protein. Thus, a 23-mer peptide (RTN1-C-CT) corresponding to residues 186-208 of RTN1-C was synthesized, and its conformation and its interaction with nucleic acids were investigated. Here we demonstrate the strong ability of RTN1-C-CT peptide to bind and condense the nucleic acids using electrophoretic and spectroscopic techniques. To determine if the binding of RTN1-C to nucleic acids could be regulated in vivo by an acetylation-deacetylation mechanism, as for the histone proteins, we studied the interaction of RTN1-C with one zinc-dependent histone deacetylase (HDAC) enzyme, HDAC8, with fluorescence and kinetic techniques using an acetylated form of RTN1-C-CT. The results reported here allow us to propose that the nucleic acid binding property of RTN1-C may have an important role in the biological function of this protein, the function of which could be regulated by an acetylation-deacetylation mechanism.
Melino, S., Nepravishta, R., Bellomaria, A., Marco, S.D., & Paci, M. (2009). Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein. BIOCHEMISTRY, 48(2), 242-253.
| Tipologia: | Articolo su rivista |
| Citazione: | Melino, S., Nepravishta, R., Bellomaria, A., Marco, S.D., & Paci, M. (2009). Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein. BIOCHEMISTRY, 48(2), 242-253. |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/10 |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/bi801407w |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 2009 |
| Titolo: | Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein |
| Autori: | |
| Autori: | Melino, S; Nepravishta, R; Bellomaria, A; Marco, SD; Paci, M |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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