RTN1-C protein is a membrane protein localized in the ER and expressed in the nervous system. Its biological role is still unclear, although interactions of the N-terminal region of RTN1-C with proteins involved in vesicle trafficking have been observed, but the role of the C-terminal region of this family protein remains to be investigated. By a homology analysis of the amino acid sequence, we identified in the C-terminal region of RTN1-C a unique consensus sequence characteristic of H4 histone protein. Thus, a 23-mer peptide (RTN1-C-CT) corresponding to residues 186-208 of RTN1-C was synthesized, and its conformation and its interaction with nucleic acids were investigated. Here we demonstrate the strong ability of RTN1-C-CT peptide to bind and condense the nucleic acids using electrophoretic and spectroscopic techniques. To determine if the binding of RTN1-C to nucleic acids could be regulated in vivo by an acetylation-deacetylation mechanism, as for the histone proteins, we studied the interaction of RTN1-C with one zinc-dependent histone deacetylase (HDAC) enzyme, HDAC8, with fluorescence and kinetic techniques using an acetylated form of RTN1-C-CT. The results reported here allow us to propose that the nucleic acid binding property of RTN1-C may have an important role in the biological function of this protein, the function of which could be regulated by an acetylation-deacetylation mechanism.
Melino, S., Nepravishta, R., Bellomaria, A., Marco, S.D., & Paci, M. (2009). Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein. BIOCHEMISTRY, 48(2), 242-253.
|Tipologia:||Articolo su rivista|
|Citazione:||Melino, S., Nepravishta, R., Bellomaria, A., Marco, S.D., & Paci, M. (2009). Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein. BIOCHEMISTRY, 48(2), 242-253.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/10|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/bi801407w|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2009|
|Titolo:||Nucleic acid binding of the RTN1-C C-terminal region: Toward the functional role of a reticulon protein|
|Autori:||Melino, S; Nepravishta, R; Bellomaria, A; Marco, SD; Paci, M|
|Appare nelle tipologie:||01 - Articolo su rivista|