Salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. A novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (ELIME array), is proposed for the detection of Salmonella in meat samples. After an optimization study, using Salmonella enterica serotype Enteritidis as reference antigen, the ability of the method to interact with a large number of Salmonella serovars commonly present in food was evaluated. The assay was then used to analyze samples of pork, chicken, beef, and turkey experimentally inoculated with Salmonella as well as real samples. The results were compared with those from the International Standard of Organization (ISO) culture method. The comparison showed that the ELIME array is able to detect a low number of Salmonella cells (1-10 CFU per 25 g) after only 6 h of incubation in a pre-enrichment broth. The investigation revealed a very good agreement between culture and ELIME array methods for meat samples, reducing the time for performing the analysis and obtaining the results quickly.

Delibato, E., Volpe, G., Romanazzo, D., De Medici, D., Toti, L., Moscone, D., et al. (2009). Development and application of an electrochemical plate coupled with immunomagnetic beads (ELIME) array for salmonella enterica detection in meat samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 57(16), 7200-7204 [10.1021/jf901181m].

Development and application of an electrochemical plate coupled with immunomagnetic beads (ELIME) array for salmonella enterica detection in meat samples

VOLPE, GIULIA;MOSCONE DINIA, DANILA;PALLESCHI, GIUSEPPE
2009

Abstract

Salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. A novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (ELIME array), is proposed for the detection of Salmonella in meat samples. After an optimization study, using Salmonella enterica serotype Enteritidis as reference antigen, the ability of the method to interact with a large number of Salmonella serovars commonly present in food was evaluated. The assay was then used to analyze samples of pork, chicken, beef, and turkey experimentally inoculated with Salmonella as well as real samples. The results were compared with those from the International Standard of Organization (ISO) culture method. The comparison showed that the ELIME array is able to detect a low number of Salmonella cells (1-10 CFU per 25 g) after only 6 h of incubation in a pre-enrichment broth. The investigation revealed a very good agreement between culture and ELIME array methods for meat samples, reducing the time for performing the analysis and obtaining the results quickly.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore CHIM/01 - Chimica Analitica
English
Con Impact Factor ISI
Elime array; Immunomagnetic beads; Meat samples; Salmonella
Delibato, E., Volpe, G., Romanazzo, D., De Medici, D., Toti, L., Moscone, D., et al. (2009). Development and application of an electrochemical plate coupled with immunomagnetic beads (ELIME) array for salmonella enterica detection in meat samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 57(16), 7200-7204 [10.1021/jf901181m].
Delibato, E; Volpe, G; Romanazzo, D; De Medici, D; Toti, L; MOSCONE DINIA, D; Palleschi, G
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
Salmonella.pdf

accesso aperto

Dimensione 743.35 kB
Formato Adobe PDF
743.35 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/25681
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 26
social impact