In the present work, we described the expression and activity of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cells (PGCs) from 8.5-14.5 days post coitum (dpc) and investigated whether these kinases play a role in regulating the various processes of PGC development. Using immunofluorescence and immunoblotting to detect the active phosphorylated form of ERK1-2 (p-ERK1-2), we found that the kinases were present in most proliferating 8.5-10.5 dpc PGCs, low in 11.5 dpc PGCs, and progressively increasing between 12.5-14.5 dpc both in female and male PGCs. In vitro culture experiments showed that inhibiting activation of ERK1-2 with the MEK-specific inhibitor U0126 significantly reduced the growth of 8.5 dpc PGCs in culture but had little effect on 11.5-12.5 dpc PGCs. Moreover, we found that the inhibitor did not affect the adhesion of 11.5 dpc PGCs, but it significantly reduced their motility features onto a cell monolayer. Further, while the ability of female PGCs to begin meiosis was not significantly affected by U0126, their progression through meiotic prophase I was slowed down. Notably, the activity of ERK1-2 was necessary for maintaining the correct expression of oocyte-specific genes crucial for germ cells survival and the formation of primordial follicles.

Sorrenti, M., Klinger, F.g., Iona, S., Rossi, V., Marcozzi, S., DE Felici, M. (2020). Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development. JOURNAL OF REPRODUCTION AND DEVELOPMENT, 66(5), 399-409 [10.1262/jrd.2019-141].

Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development

Sorrenti, Maria;Klinger, Francesca Gioia
Investigation
;
Marcozzi, Serena;DE Felici, Massimo
2020-10-13

Abstract

In the present work, we described the expression and activity of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cells (PGCs) from 8.5-14.5 days post coitum (dpc) and investigated whether these kinases play a role in regulating the various processes of PGC development. Using immunofluorescence and immunoblotting to detect the active phosphorylated form of ERK1-2 (p-ERK1-2), we found that the kinases were present in most proliferating 8.5-10.5 dpc PGCs, low in 11.5 dpc PGCs, and progressively increasing between 12.5-14.5 dpc both in female and male PGCs. In vitro culture experiments showed that inhibiting activation of ERK1-2 with the MEK-specific inhibitor U0126 significantly reduced the growth of 8.5 dpc PGCs in culture but had little effect on 11.5-12.5 dpc PGCs. Moreover, we found that the inhibitor did not affect the adhesion of 11.5 dpc PGCs, but it significantly reduced their motility features onto a cell monolayer. Further, while the ability of female PGCs to begin meiosis was not significantly affected by U0126, their progression through meiotic prophase I was slowed down. Notably, the activity of ERK1-2 was necessary for maintaining the correct expression of oocyte-specific genes crucial for germ cells survival and the formation of primordial follicles.
13-ott-2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
Extracellular signal-regulated kinases (ERKs)
Gene expression
Meiosis
Oogenesis
Primordial germ cells
https://www.jstage.jst.go.jp/article/jrd/66/5/66_2019-141/_article
10.1262/jrd.2019-141
Sorrenti, M., Klinger, F.g., Iona, S., Rossi, V., Marcozzi, S., DE Felici, M. (2020). Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development. JOURNAL OF REPRODUCTION AND DEVELOPMENT, 66(5), 399-409 [10.1262/jrd.2019-141].
Sorrenti, M; Klinger, Fg; Iona, S; Rossi, V; Marcozzi, S; DE Felici, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/255305
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