Electrospinning is a valuable technique to fabricate fibrous scaffolds for tissue engineering. The typical nonwoven architecture allows cell adhesion and proliferation, and supports diffusion of nutrients and waste products. Poly(F-caprolactone) (PCL) electrospun membranes were produced starting from 14% w/v solutions in (a) mixture 1:1 tetrahydrofuran and N,N-dimethylformamide and (b) chloroform. Matrices made up of randomly arranged uniform fibers free of beads were obtained. The average fiber diameters were (a) 0.8 +/- 0.2 mu m and (b) 3.6 +/- 0.8 pm. PCL matrices showed the following tensile mechanical properties: tensile modulus (a) 5.0 +/- 0.7 MPa (b) 6.4 +/- 0.2 MPa, yield stress (a) 0.55 +/- 0.06 MPa (b) 0.43 +/- 0.02 MPa, and ultimate tensile stress (a) 1.7 +/- 0.2 MPa and (b) 0.8 +/- 0.1 MPa. The ultimate strain ranged between 300% and 400%. Cytotoxicity of electrospun membranes was continuously evaluated by means of electric cell-substrate impedance sensing technique using human umbilical vein endothelial cells (HUVEC). PCL matrices resulted free of toxic amounts of contaminants and/or process by-products. In vitro studies performed by culturing HUVEC on micrometric and submicrometric fibrous mats showed that both structures supported cell adhesion and spreading. However, cells cultured on the micrometric network showed higher vitality and improved interaction with the polymeric fibers, suggesting an increased ability to promote cell colonization. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 1028-1039, 2009

Del Gaudio, C., Bianco, A., Folin, M., Baiguera, S., & Grigioni, M. (2009). Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, 89(4), 1028-1039 [10.1002/jbm.a.32048].

Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers

BIANCO, ALESSANDRA;
2009

Abstract

Electrospinning is a valuable technique to fabricate fibrous scaffolds for tissue engineering. The typical nonwoven architecture allows cell adhesion and proliferation, and supports diffusion of nutrients and waste products. Poly(F-caprolactone) (PCL) electrospun membranes were produced starting from 14% w/v solutions in (a) mixture 1:1 tetrahydrofuran and N,N-dimethylformamide and (b) chloroform. Matrices made up of randomly arranged uniform fibers free of beads were obtained. The average fiber diameters were (a) 0.8 +/- 0.2 mu m and (b) 3.6 +/- 0.8 pm. PCL matrices showed the following tensile mechanical properties: tensile modulus (a) 5.0 +/- 0.7 MPa (b) 6.4 +/- 0.2 MPa, yield stress (a) 0.55 +/- 0.06 MPa (b) 0.43 +/- 0.02 MPa, and ultimate tensile stress (a) 1.7 +/- 0.2 MPa and (b) 0.8 +/- 0.1 MPa. The ultimate strain ranged between 300% and 400%. Cytotoxicity of electrospun membranes was continuously evaluated by means of electric cell-substrate impedance sensing technique using human umbilical vein endothelial cells (HUVEC). PCL matrices resulted free of toxic amounts of contaminants and/or process by-products. In vitro studies performed by culturing HUVEC on micrometric and submicrometric fibrous mats showed that both structures supported cell adhesion and spreading. However, cells cultured on the micrometric network showed higher vitality and improved interaction with the polymeric fibers, suggesting an increased ability to promote cell colonization. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 1028-1039, 2009
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore ING-IND/22 - Scienza e Tecnologia dei Materiali
English
Con Impact Factor ISI
Electric cell-substrate impedance sensing (ECIS); Electrospinning; HUVEC; Morphological study; Poly(e-caprolactone) (PCL); Tissue engineering
Del Gaudio, C., Bianco, A., Folin, M., Baiguera, S., & Grigioni, M. (2009). Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, 89(4), 1028-1039 [10.1002/jbm.a.32048].
Del Gaudio, C; Bianco, A; Folin, M; Baiguera, S; Grigioni, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/25476
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