We report here the results of elastic incoherent neutron scattering experiments on three globular proteins (trypsin, lysozyme and beta-lactoglobulin) in different pressure intervals ranging from 1 bar to 5.5 kbar. A decrease of the mean square hydrogen fluctuations, u(2), has been observed upon increasing pressure. Trypsin and beta-lactoglobulin behave similarly while lysozyme shows much larger changes in u(2). This can be related to different steps in the denaturing processes and to the high propensity of lysozyme to form amyloids. Elastic incoherent neutron scattering has proven to be an effective microscopic technique for the investigation of pressure induced changes in protein flexibility.

Filabozzi, A., Deriu, A., Di Bari, M., Russo, D., Croci, S., DI VENERE, A. (2010). Elastic incoherent neutron scattering as a probe of high pressure induces changes in protein flexibility. BIOCHIMICA ET BIOPHYSICA ACTA, 1804, 63-67 [10.1016/j.bbapap.2009.08.025].

Elastic incoherent neutron scattering as a probe of high pressure induces changes in protein flexibility

FILABOZZI, ALESSANDRA;DI VENERE, ALMERINDA
2010-01-01

Abstract

We report here the results of elastic incoherent neutron scattering experiments on three globular proteins (trypsin, lysozyme and beta-lactoglobulin) in different pressure intervals ranging from 1 bar to 5.5 kbar. A decrease of the mean square hydrogen fluctuations, u(2), has been observed upon increasing pressure. Trypsin and beta-lactoglobulin behave similarly while lysozyme shows much larger changes in u(2). This can be related to different steps in the denaturing processes and to the high propensity of lysozyme to form amyloids. Elastic incoherent neutron scattering has proven to be an effective microscopic technique for the investigation of pressure induced changes in protein flexibility.
2010
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA)
English
Con Impact Factor ISI
Filabozzi, A., Deriu, A., Di Bari, M., Russo, D., Croci, S., DI VENERE, A. (2010). Elastic incoherent neutron scattering as a probe of high pressure induces changes in protein flexibility. BIOCHIMICA ET BIOPHYSICA ACTA, 1804, 63-67 [10.1016/j.bbapap.2009.08.025].
Filabozzi, A; Deriu, A; Di Bari, M; Russo, D; Croci, S; DI VENERE, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/25169
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