Adhesion is a key process when ultrasound contrast agents, i.e. microbubbles, approach pathological tissues. A way to accomplish tumour targeting is to tether surface engineered microbubbles to endothelial cells of the up-regulated vascularization of cancer tissues. This can be achieved by coupling the microbubbles surface with the Arginine-Glycine-Aspartate, RGD, sequence. Such molecule interacts with the integrin receptors placed on the endothelial cells. Stability and trajectories of RGD modified lipid shelled MBs have been analysed in vitro using microchannels coated with human umbilical vein endothelial cells, HUVEC. In the microchannels realistic conditions, close to the physiological ones, were reproduced replicating shear rate, roughness comparable to the endothelium and channel size mimicking the postcapillary venules. In these conditions, the analysis of the trajectories close to the walls highlights a substantial difference between the modified MBs and the plain ones. Moreover, MBs adhesion has dynamic features recalling the motion of neutrophils engaged near the substrate such as rolling, translations and transient detachments. These findings are useful for the optimization of in vivo imaging and targeting functions.

Cerroni, B., Righi Riva, F., Oddo, L., Domenici, F., Tortorella, E., Toumia, Y., et al. (2020). In vitro analysis of the trajectories of adhesive microbubbles approaching endothelial cells. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 578, 758-767 [10.1016/j.jcis.2020.06.009].

In vitro analysis of the trajectories of adhesive microbubbles approaching endothelial cells

B. Cerroni;L. Oddo;F. Domenici;Y. Toumia;G. Paradossi
2020-06

Abstract

Adhesion is a key process when ultrasound contrast agents, i.e. microbubbles, approach pathological tissues. A way to accomplish tumour targeting is to tether surface engineered microbubbles to endothelial cells of the up-regulated vascularization of cancer tissues. This can be achieved by coupling the microbubbles surface with the Arginine-Glycine-Aspartate, RGD, sequence. Such molecule interacts with the integrin receptors placed on the endothelial cells. Stability and trajectories of RGD modified lipid shelled MBs have been analysed in vitro using microchannels coated with human umbilical vein endothelial cells, HUVEC. In the microchannels realistic conditions, close to the physiological ones, were reproduced replicating shear rate, roughness comparable to the endothelium and channel size mimicking the postcapillary venules. In these conditions, the analysis of the trajectories close to the walls highlights a substantial difference between the modified MBs and the plain ones. Moreover, MBs adhesion has dynamic features recalling the motion of neutrophils engaged near the substrate such as rolling, translations and transient detachments. These findings are useful for the optimization of in vivo imaging and targeting functions.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02
English
Microbubbles, Adhesion, Rolling, Targeting, RGD, Integrins
Cerroni, B., Righi Riva, F., Oddo, L., Domenici, F., Tortorella, E., Toumia, Y., et al. (2020). In vitro analysis of the trajectories of adhesive microbubbles approaching endothelial cells. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 578, 758-767 [10.1016/j.jcis.2020.06.009].
Cerroni, B; Righi Riva, F; Oddo, L; Domenici, F; Tortorella, E; Toumia, Y; Brasili, F; Paradossi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/250752
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