A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 mu M by using luminol and 4-iodophenol as enhancer agent.

Costantini, F., Tiggelaar, R.m., Salvio, R., Nardecchia, M., Schlautmann, S., Manetti, C., et al. (2017). An all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay. BIOSENSORS, 7(4), 58 [10.3390/bios7040058].

An all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay

Salvio R.;
2017-01-01

Abstract

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 mu M by using luminol and 4-iodophenol as enhancer agent.
2017
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/06 - CHIMICA ORGANICA
English
Con Impact Factor ISI
amorphous silicon photosensors; chemiluminescent based assay; lab-on-chip; microfluidic channel; surface functionalization; Enzyme Assays; Equipment Design; Luminescent Measurements; Biosensing Techniques; Glass; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques; Microfluidics; Photochemical Processes; Silicon
Costantini, F., Tiggelaar, R.m., Salvio, R., Nardecchia, M., Schlautmann, S., Manetti, C., et al. (2017). An all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay. BIOSENSORS, 7(4), 58 [10.3390/bios7040058].
Costantini, F; Tiggelaar, Rm; Salvio, R; Nardecchia, M; Schlautmann, S; Manetti, C; Gardeniers, Hjge; de Cesare, G; Caputo, D; Nascetti, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/247680
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