Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting N500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients.Genotypingwas performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres.Mixtures of STR markers (i.e., >= 2 alleles for >= 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15).Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients. (C) 2017 The Authors. Published by Elsevier B.V.

Alanio, A., Gits-Muselli, M., Guigue, N., Desnos-Ollivier, M., Calderon, E.J., Di Cave, D., et al. (2017). Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study. EBIOMEDICINE, 22, 155-163 [10.1016/j.ebiom.2017.06.027].

Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Di Cave D.;
2017

Abstract

Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting N500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients.Genotypingwas performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres.Mixtures of STR markers (i.e., >= 2 alleles for >= 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15).Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients. (C) 2017 The Authors. Published by Elsevier B.V.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06
English
Europe; Genotyping; MLS typing; Microsatellites; Mixed infection; Pneumocystis jirovecii; Transmission; Adult; Aged; DNA, Fungal; Europe; Female; Genetic Variation; Genotyping Techniques; Humans; Male; Microsatellite Repeats; Middle Aged; Phylogeny; Phylogeography; Pneumocystis carinii; Pneumonia, Pneumocystis
Alanio, A., Gits-Muselli, M., Guigue, N., Desnos-Ollivier, M., Calderon, E.J., Di Cave, D., et al. (2017). Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study. EBIOMEDICINE, 22, 155-163 [10.1016/j.ebiom.2017.06.027].
Alanio, A; Gits-Muselli, M; Guigue, N; Desnos-Ollivier, M; Calderon, Ej; Di Cave, D; Dupont, D; Hamprecht, A; Hauser, Pm; Helweg-Larsen, J; Kicia, M; Lagrou, K; Lengerova, M; Matos, O; Melchers, Wjg; Morio, F; Nevez, G; Totet, A; White, Lp; Bretagne, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/247645
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