β -lactoglobulin aggregates are used as structuring agents in food processing. Their enzymatic degradation follows different pathways depending on the enzyme and the pH. The transient species produced during the degradation process are difficult to characterize under continuous measurement. Here, conical track-etched nanopores are used to investigate the β -lactoglobulin degradation by pepsin (pH 2) and trypsin (pH 9). Before enzyme addition, two distinct populations, oligomers and protofibrils, are identified. Just after enzyme addition, the aggregate size decreases. For pepsin, a phenomenon of reaggregation that does not occur for the trypsin is evidenced. In addition, after 140 min a larger population of aggregate appears. The experimental results are supported by a kinetic model. This work also demonstrates that conical nanopore allows following the kinetic of transient protein aggregate intermediates during enzymatic degradation.

Giamblanco, N., Janot, J.-., Gubbiotti, A., Chinappi, M., Balme, S. (2020). Characterization of food amyloid protein digestion by conical nanopore. SMALL METHODS, 4(11) [10.1002/smtd.201900703].

Characterization of food amyloid protein digestion by conical nanopore

Chinappi M.;
2020-01-01

Abstract

β -lactoglobulin aggregates are used as structuring agents in food processing. Their enzymatic degradation follows different pathways depending on the enzyme and the pH. The transient species produced during the degradation process are difficult to characterize under continuous measurement. Here, conical track-etched nanopores are used to investigate the β -lactoglobulin degradation by pepsin (pH 2) and trypsin (pH 9). Before enzyme addition, two distinct populations, oligomers and protofibrils, are identified. Just after enzyme addition, the aggregate size decreases. For pepsin, a phenomenon of reaggregation that does not occur for the trypsin is evidenced. In addition, after 140 min a larger population of aggregate appears. The experimental results are supported by a kinetic model. This work also demonstrates that conical nanopore allows following the kinetic of transient protein aggregate intermediates during enzymatic degradation.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ING-IND/06 - FLUIDODINAMICA
Settore ING-IND/34 - BIOINGEGNERIA INDUSTRIALE
English
Giamblanco, N., Janot, J.-., Gubbiotti, A., Chinappi, M., Balme, S. (2020). Characterization of food amyloid protein digestion by conical nanopore. SMALL METHODS, 4(11) [10.1002/smtd.201900703].
Giamblanco, N; Janot, J-; Gubbiotti, A; Chinappi, M; Balme, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/247351
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