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N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).

Fezza, F., Mastrangelo, N., Maccarrone, M. (2016). Assay of NAPE-PLD Activity. In Mauro Maccarrone (a cura di), “Endocannabinoid Signaling: Methods and Protocols” (pp. 123-130). Springer [10.1007/978-1-4939-3539-0_13].

Assay of NAPE-PLD Activity

Fezza, Filomena;Mastrangelo, Nicolina;Maccarrone, Mauro
2016-01-01

Abstract

N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).
2016
Settore BIO/10 - BIOCHIMICA
English
Rilevanza internazionale
Capitolo o saggio
Anandamide; Fatty acid amides; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; N-acyl-phosphatidylethanolamines; N-palmitoylethanolamine; Radioactive assay; Thin-layer chromatography; Cell Line; Chromatography, Thin Layer; Enzyme Activation; Isotope Labeling; Phosphatidylethanolamines; Phospholipase D; Substrate Specificity; Enzyme Assays
Fezza, F., Mastrangelo, N., Maccarrone, M. (2016). Assay of NAPE-PLD Activity. In Mauro Maccarrone (a cura di), “Endocannabinoid Signaling: Methods and Protocols” (pp. 123-130). Springer [10.1007/978-1-4939-3539-0_13].
Fezza, F; Mastrangelo, N; Maccarrone, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/246921
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