Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.

Bellich, B., Lagatolla, C., Rizzo, R., D'Andrea, M.m., Rossolini, G.m., Cescutti, P. (2020). Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 155, 315-323 [10.1016/j.ijbiomac.2020.03.196].

Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions

D'Andrea M. M.;
2020-01-01

Abstract

Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Settore BIO/19 - MICROBIOLOGIA GENERALE
English
Con Impact Factor ISI
Capsular polysaccharide structure; Glycosyltransferases; Klebsiella pneumoniae KPB-1; NMR
Bellich, B., Lagatolla, C., Rizzo, R., D'Andrea, M.m., Rossolini, G.m., Cescutti, P. (2020). Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 155, 315-323 [10.1016/j.ijbiomac.2020.03.196].
Bellich, B; Lagatolla, C; Rizzo, R; D'Andrea, Mm; Rossolini, Gm; Cescutti, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/245127
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