Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.

Porchetta, A., Idili, A., Vallee-Belisle, A., Ricci, F. (2015). General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands. NANO LETTERS, 15(7), 4467-4471 [10.1021/acs.nanolett.5b00852].

General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands

Porchetta A.;Idili A.;Ricci F.
2015-01-01

Abstract

Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.
2015
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
DNA nanotechnology; machines; molecular devices; Nucleic acid; pH; triplex; Aptamers, Nucleotide; Biosensing Techniques; Cocaine; DNA; DNA Probes; Delayed-Action Preparations; Fluorescent Dyes; Hydrogen-Ion Concentration; Ligands; Nanotechnology; Nucleic Acid Conformation
Porchetta, A., Idili, A., Vallee-Belisle, A., Ricci, F. (2015). General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands. NANO LETTERS, 15(7), 4467-4471 [10.1021/acs.nanolett.5b00852].
Porchetta, A; Idili, A; Vallee-Belisle, A; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/243603
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