Cerium oxide nanoparticles (nanoceria) display antioxidant properties and have shown cytoprotective effects both in vitro and in vivo. Here, we explored the effects of nanoceria on neural progenitor cells using the C17.2 murine cell line as a model. First, we assessed the effects of nanoceria versus samarium (Sm) doped nanoceria on cell viability in the presence of the prooxidant, DMNQ. Both particles were taken up by cells and nanoceria, but not Sm-doped nanoceria, elicited a temporary cytoprotective effect upon exposure to DMNQ. Next, we employed RNA sequencing to explore the transcriptional responses induced by nanoceria or Sm-doped nanoceria during neuronal differentiation. Detailed computational analyses showed that nanoceria altered pathways and networks relevant for neuronal development, leading us to hypothesize that nanoceria inhibits neuronal differentiation, and that nanoceria and Sm-doped nanoceria both interfere with cytoskeletal organization. We confirmed that nanoceria reduced neuron specific beta 3-tubulin expression, a marker of neuronal differentiation, and GFAP, a neuroglial marker. Furthermore, using super-resolution microscopy approaches, we could show that both particles interfered with cytoskeletal organization and altered the structure of neural growth cones. Taken together, these results reveal that nanoceria may impact on neuronal differentiation, suggesting that nanoceria could pose a developmental neurotoxicity hazard.
Gliga, A.R., Edoff, K., Caputo, F., Kallman, T., Blom, H., Karlsson, H.L., et al. (2017). Cerium oxide nanoparticles inhibit differentiation of neural stem cells. SCIENTIFIC REPORTS, 7(1), 9284 [10.1038/s41598-017-09430-8].
|Tipologia:||Articolo su rivista|
|Citazione:||Gliga, A.R., Edoff, K., Caputo, F., Kallman, T., Blom, H., Karlsson, H.L., et al. (2017). Cerium oxide nanoparticles inhibit differentiation of neural stem cells. SCIENTIFIC REPORTS, 7(1), 9284 [10.1038/s41598-017-09430-8].|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-017-09430-8|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2017|
|Titolo:||Cerium oxide nanoparticles inhibit differentiation of neural stem cells|
|Autori:||Gliga, AR; Edoff, K; Caputo, F; Kallman, T; Blom, H; Karlsson, HL; Ghibelli, L; Traversa, E; Ceccatelli, S; Fadeel, B|
|Appare nelle tipologie:||01 - Articolo su rivista|