Human pluripotent stem cells provide a versatile platform for regenerative studies, drug testing and disease modeling. That the expression of only four transcription factors, Oct4, Klf4, Sox2 and c-Myc (OKSM), is sufficient for generation of induced pluripotent stem cells (iPSCs) from differentiated somatic cells has revolutionized the field and also highlighted the importance of OKSM as targets for genome editing. A number of novel genome-editing systems have been developed recently. In this review, we focus on successful applications of several such systems for generation of iPSCs. In particular, we discuss genome-editing systems based on zinc-finger fusion proteins (ZFs), transcription activator-like effectors (TALEs) and an RNA-guided DNA-specific nuclease, Cas9, derived from the bacterial defense system against viruses that utilizes clustered regularly interspaced short palindromic repeats (CRISPR).

Vasileva, E.a., Shuvalov, O.u., Garabadgiu, A.v., Melino, G., Barlev, N.a. (2015). Genome-editing tools for stem cell biology. CELL DEATH & DISEASE, 6, e1831 [10.1038/cddis.2015.167].

Genome-editing tools for stem cell biology

Melino, G.;
2015-01-01

Abstract

Human pluripotent stem cells provide a versatile platform for regenerative studies, drug testing and disease modeling. That the expression of only four transcription factors, Oct4, Klf4, Sox2 and c-Myc (OKSM), is sufficient for generation of induced pluripotent stem cells (iPSCs) from differentiated somatic cells has revolutionized the field and also highlighted the importance of OKSM as targets for genome editing. A number of novel genome-editing systems have been developed recently. In this review, we focus on successful applications of several such systems for generation of iPSCs. In particular, we discuss genome-editing systems based on zinc-finger fusion proteins (ZFs), transcription activator-like effectors (TALEs) and an RNA-guided DNA-specific nuclease, Cas9, derived from the bacterial defense system against viruses that utilizes clustered regularly interspaced short palindromic repeats (CRISPR).
2015
Pubblicato
Rilevanza internazionale
Recensione
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Vasileva, E.a., Shuvalov, O.u., Garabadgiu, A.v., Melino, G., Barlev, N.a. (2015). Genome-editing tools for stem cell biology. CELL DEATH & DISEASE, 6, e1831 [10.1038/cddis.2015.167].
Vasileva, Ea; Shuvalov, Ou; Garabadgiu, Av; Melino, G; Barlev, Na
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/240240
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 13
social impact