In the present study, biochar from brewers’ spent grain was used, for the first time, to develop screen-printed electrodes. After having investigated the dispersion behaviour of biochar in different organic solvents, a biochar-based screen-printed electrode was prepared with the drop-casting technique. In order to understand the electrochemical potentiality and performances of the biochar/sensor tool, different electroactive species, i.e., ferricyanide, benzoquinone, epinephrine, ascorbic, and uric acids, were used. The results were compared with those of the same electrodes that were modified with commercial graphene, confirming that the proposed electrode showed improved electrochemical behaviour in terms of resolution, peak-to-peak separation, current intensity, and resistance to charge transfer. Furthermore, a tyrosinase biosensor was developed by direct immobilisation of this enzyme on the biochar/screen printed electrode, as an example of the potential of biochar for disposable biosensor development. The efficiently occurred immobilisation of the biochar on the screen printed electrode’s (SPE’s) surface was demonstrated by the observation of the working electrode with a scanning electron microscope. The detection was performed by measuring the current due to the reduction of the corresponding quinone at low potential, equal to −0.310 V for epinephrine. The experimental conditions for the tyrosinase immobilization and the analytical parameters, such as applied potential and pH of buffer, were studied and optimized. Under these conditions, the electrochemical biosensors were characterized. A linear working range of epinephrine was obtained from 0.05 up to 0.5 mM. The detection limit was 2 × 10−4 mM for the biosensor.

Cancelliere, R., Carbone, K., Pagano, M., Cacciotti, I., Micheli, L. (2019). Biochar from Brewers' Spent Grain: a green and low-cost smart material to modify screen-printed electrodes. BIOSENSORS, 9(4), 139-155 [10.3390/bios9040139].

Biochar from Brewers' Spent Grain: a green and low-cost smart material to modify screen-printed electrodes

Cacciotti Ilaria;Micheli Laura
2019-12-01

Abstract

In the present study, biochar from brewers’ spent grain was used, for the first time, to develop screen-printed electrodes. After having investigated the dispersion behaviour of biochar in different organic solvents, a biochar-based screen-printed electrode was prepared with the drop-casting technique. In order to understand the electrochemical potentiality and performances of the biochar/sensor tool, different electroactive species, i.e., ferricyanide, benzoquinone, epinephrine, ascorbic, and uric acids, were used. The results were compared with those of the same electrodes that were modified with commercial graphene, confirming that the proposed electrode showed improved electrochemical behaviour in terms of resolution, peak-to-peak separation, current intensity, and resistance to charge transfer. Furthermore, a tyrosinase biosensor was developed by direct immobilisation of this enzyme on the biochar/screen printed electrode, as an example of the potential of biochar for disposable biosensor development. The efficiently occurred immobilisation of the biochar on the screen printed electrode’s (SPE’s) surface was demonstrated by the observation of the working electrode with a scanning electron microscope. The detection was performed by measuring the current due to the reduction of the corresponding quinone at low potential, equal to −0.310 V for epinephrine. The experimental conditions for the tyrosinase immobilization and the analytical parameters, such as applied potential and pH of buffer, were studied and optimized. Under these conditions, the electrochemical biosensors were characterized. A linear working range of epinephrine was obtained from 0.05 up to 0.5 mM. The detection limit was 2 × 10−4 mM for the biosensor.
dic-2019
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
Settore AGR/13 - CHIMICA AGRARIA
English
Con Impact Factor ISI
brewers’ spent grain; biochar; screen-printed electrode; biosensor; tyrosinase
Cancelliere, R., Carbone, K., Pagano, M., Cacciotti, I., Micheli, L. (2019). Biochar from Brewers' Spent Grain: a green and low-cost smart material to modify screen-printed electrodes. BIOSENSORS, 9(4), 139-155 [10.3390/bios9040139].
Cancelliere, R; Carbone, K; Pagano, M; Cacciotti, I; Micheli, L
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
biosensors-09-00139-v2.pdf

accesso aperto

Licenza: Copyright dell'editore
Dimensione 5.57 MB
Formato Adobe PDF
5.57 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/234719
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 39
social impact