The objective of the present work was the development of a micro-pH meter for the determination of the pH value within bioreactors with a volume of up to 200 μl in total. Two different prototypes of optodes were designed and tested. In a first approach spectroscopic analysis of bromothymol blue in a micro-sized-channel structure was carried out utilizing glass fibers, enabling measurements in sample volumes down to the range of picoliters. In a second approach a different illumination system consisting of a RGB-sensor and a LED light source was used. Phenol red was successfully applied as the pH indicator for this setup

Rogge, C., Zinn, S., Schneider, S., Francini, R., Prosposito, P., Foitzik, A. (2017). A new approach for the spectroscopic detection of different pH-values. In Materials Fcience Forum (pp. 1606-1611). Trans Tech Publications Ltd [10.4028/www.scientific.net/MSF.879.1606].

A new approach for the spectroscopic detection of different pH-values

Rogge C.;Francini R.;Prosposito P.;
2017-01-01

Abstract

The objective of the present work was the development of a micro-pH meter for the determination of the pH value within bioreactors with a volume of up to 200 μl in total. Two different prototypes of optodes were designed and tested. In a first approach spectroscopic analysis of bromothymol blue in a micro-sized-channel structure was carried out utilizing glass fibers, enabling measurements in sample volumes down to the range of picoliters. In a second approach a different illumination system consisting of a RGB-sensor and a LED light source was used. Phenol red was successfully applied as the pH indicator for this setup
2017
Settore FIS/03 - FISICA DELLA MATERIA
Settore ING-IND/22 - SCIENZA E TECNOLOGIA DEI MATERIALI
Settore ING-IND/23 - CHIMICA FISICA APPLICATA
English
Rilevanza internazionale
Articolo scientifico in atti di convegno
Rogge, C., Zinn, S., Schneider, S., Francini, R., Prosposito, P., Foitzik, A. (2017). A new approach for the spectroscopic detection of different pH-values. In Materials Fcience Forum (pp. 1606-1611). Trans Tech Publications Ltd [10.4028/www.scientific.net/MSF.879.1606].
Rogge, C; Zinn, S; Schneider, S; Francini, R; Prosposito, P; Foitzik, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/233875
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