The objective of the present work was the development of a micro-pH meter for the determination of the pH value within bioreactors with a volume of up to 200 μl in total. Two different prototypes of optodes were designed and tested. In a first approach spectroscopic analysis of bromothymol blue in a micro-sized-channel structure was carried out utilizing glass fibers, enabling measurements in sample volumes down to the range of picoliters. In a second approach a different illumination system consisting of a RGB-sensor and a LED light source was used. Phenol red was successfully applied as the pH indicator for this setup
Rogge, C., Zinn, S., Schneider, S., Francini, R., Prosposito, P., Foitzik, A. (2017). A new approach for the spectroscopic detection of different pH-values. In Materials Fcience Forum (pp. 1606-1611). Trans Tech Publications Ltd [10.4028/www.scientific.net/MSF.879.1606].
A new approach for the spectroscopic detection of different pH-values
Rogge C.;Francini R.;Prosposito P.;
2017-01-01
Abstract
The objective of the present work was the development of a micro-pH meter for the determination of the pH value within bioreactors with a volume of up to 200 μl in total. Two different prototypes of optodes were designed and tested. In a first approach spectroscopic analysis of bromothymol blue in a micro-sized-channel structure was carried out utilizing glass fibers, enabling measurements in sample volumes down to the range of picoliters. In a second approach a different illumination system consisting of a RGB-sensor and a LED light source was used. Phenol red was successfully applied as the pH indicator for this setupI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
