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EnglishRNA metabolism is tightly controlled across different tissues and developmental stages, and its dysregulation is one of the molecular hallmarks of cancer. Through direct binding to specific sequence element(s), RNA binding proteins (RBPs) play a pivotal role in co-and post-transcriptional RNA regulatory events. We have recently demonstrated that, in pancreatic cancer cells, acquisition of a drug resistant (DR)-phenotype relied on upregulation of the polypyrimidine tract binding protein (PTBP1), which in turn is recruited to the pyruvate kinase pre-mRNA and favors splicing of the oncogenic PKM2 variant. Herein, we describe a step-by-step protocol of the ultraviolet (UV) light cross-linking and immunoprecipitation (CLIP) method to determine the direct binding of an RBP to specific regions of its target RNAs in adherent human cell lines.
Bielli, P., & Sette, C. (2017). Analysis of in vivo Interaction between RNA Binding Proteins and Their RNA Targets by UV Cross-linking and Immunoprecipitation (CLIP) Method. BIO-PROTOCOL, 7(10).
| Tipologia: | Articolo su rivista |
| Citazione: | Bielli, P., & Sette, C. (2017). Analysis of in vivo Interaction between RNA Binding Proteins and Their RNA Targets by UV Cross-linking and Immunoprecipitation (CLIP) Method. BIO-PROTOCOL, 7(10). |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/16 |
| Revisione (peer review): | Esperti anonimi |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.21769/BioProtoc.2274 |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 20-mag-2017 |
| Titolo: | Analysis of in vivo Interaction between RNA Binding Proteins and Their RNA Targets by UV Cross-linking and Immunoprecipitation (CLIP) Method |
| Autori: | |
| Autori: | Bielli, P; Sette, C |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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http://hdl.handle.net/2108/231904
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