Bacteria adapt their pattern of gene expression in response to a variety of external cues, including fluctuations in population density. This type of bacterial cell-to-cell communication is referred to as quorum-sensing. Quorum-sensing systems are present in many bacterial species and constitute a large collection of ligands and cognate receptors. The availability of such diversity offers interesting opportunities for biotechnological exploitation. We describe here the transformation of the quorum-sensing system of Agrobacterium tumefaciens into a transcription regulatory system that works in mammalian cells. The A. tumefaciens TraR protein was fused to the eukaryotic activation domain of NF-kappaB p65, generating a novel chimaeric transcriptional activator that stimulates gene transcription in different human cell lines from a minimal promoter containing the TraR DNA recognition sequence in the presence of the Agrobacterium quorum-sensing signal molecule N-(3-oxo-octanoyl)homoserine lactone (3-oxo-C-8-HSL). The basal level of transcription was low in the absence of 3-oxo-C-8-HSL, and gene expression was stimulated up to 1,000-fold at a saturating concentration of 3-oxo-C-8-HSL.

Neddermann, P., Gargioli, C., Muraglia, E., Sambucini, S., Bonelli, F., De Francesco, R., et al. (2003). A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR. EMBO REPORTS, 4(2), 159-165 [10.1038/sj.embor.embor734].

A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR

Gargioli C.;
2003-02-01

Abstract

Bacteria adapt their pattern of gene expression in response to a variety of external cues, including fluctuations in population density. This type of bacterial cell-to-cell communication is referred to as quorum-sensing. Quorum-sensing systems are present in many bacterial species and constitute a large collection of ligands and cognate receptors. The availability of such diversity offers interesting opportunities for biotechnological exploitation. We describe here the transformation of the quorum-sensing system of Agrobacterium tumefaciens into a transcription regulatory system that works in mammalian cells. The A. tumefaciens TraR protein was fused to the eukaryotic activation domain of NF-kappaB p65, generating a novel chimaeric transcriptional activator that stimulates gene transcription in different human cell lines from a minimal promoter containing the TraR DNA recognition sequence in the presence of the Agrobacterium quorum-sensing signal molecule N-(3-oxo-octanoyl)homoserine lactone (3-oxo-C-8-HSL). The basal level of transcription was low in the absence of 3-oxo-C-8-HSL, and gene expression was stimulated up to 1,000-fold at a saturating concentration of 3-oxo-C-8-HSL.
feb-2003
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13 - BIOLOGIA APPLICATA
English
Bacterial Proteins; DNA; Gene Expression; Gene Expression Regulation; Protein Structure, Tertiary; Recombinant Fusion Proteins; Transcription, Genetic
Neddermann, P., Gargioli, C., Muraglia, E., Sambucini, S., Bonelli, F., De Francesco, R., et al. (2003). A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR. EMBO REPORTS, 4(2), 159-165 [10.1038/sj.embor.embor734].
Neddermann, P; Gargioli, C; Muraglia, E; Sambucini, S; Bonelli, F; De Francesco, R; Cortese, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/228904
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