The manufacturing of artificial bone grafts can potentially circumvent the issues associated with current bone grafting treatments for critical-size bone defects caused by pathological disorders, trauma, or massive tumor ablation. In this study, we report on a potentially patient-specific fabrication process in which replicas of bone defects, in particular zygomatic and mandibular bones and phalanxes of a hand finger, were manufactured by laser stereolithography and used as templates for the creation of PDMS molds. Gas-in-water foams were cast in the molds, rapidly frozen, freeze-dried, and cross-linked. Since bone matrix consists essentially of collagen and hydroxyapatite, biomimetic scaffolds were fabricated using gelatin and hydroxyapatite in a ratio very similar to that found in bone. The obtained composite scaffolds were excellent replicas of the original bone defects models and presented both a superficial and internal porous texture adequate for cellular and blood vessels infiltration. In particular, scaffolds exhibited a porous texture consisting of pores and interconnects with average size of about 300 and 100 μm, respectively, and a porosity of 90%. In vitro culture tests using hMSCs demonstrated scaffold biocompatibility and capacity in inducing differentiation toward osteoblasts progenitors. In vivo cellularized implants showed bone matrix deposition and recruitment of blood vessels. Overall, the technique/materials combination used in this work led to the fabrication of promising mechanically stable, bioactive, and biocompatible composite scaffolds with well-defined architectures potentially valuable in the regeneration of patient-specific bone defects.
Cianciosi, A., Costantini, M., Bergamasco, S., Testa, S., Fornetti, E., Jaroszewicz, J., et al. (2019). Engineering Human-Scale Artificial Bone Grafts for Treating Critical-Size Bone Defects. ACS APPLIED BIO MATERIALS, 2(11), 5077-5092.
|Tipologia:||Articolo su rivista|
|Citazione:||Cianciosi, A., Costantini, M., Bergamasco, S., Testa, S., Fornetti, E., Jaroszewicz, J., et al. (2019). Engineering Human-Scale Artificial Bone Grafts for Treating Critical-Size Bone Defects. ACS APPLIED BIO MATERIALS, 2(11), 5077-5092.|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/acsabm.9b00756|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2019|
|Titolo:||Engineering Human-Scale Artificial Bone Grafts for Treating Critical-Size Bone Defects|
|Autori:||Cianciosi, A; Costantini, M; Bergamasco, S; Testa, S; Fornetti, E; Jaroszewicz, J; Baldi, J; Latini, A; Choinska, E; Heljak, M; Zoccali, C; Cannata, S; Swieszkowski, W; Diaz Lantada, A; Gargioli, C; Barbetta, A|
|Appare nelle tipologie:||01 - Articolo su rivista|