The cannabinoid CB 2 receptor (CB 2 R) represents a promising therapeutic target for various forms of tissue injury and inflammatory diseases. Although numerous compounds have been developed and widely used to target CB 2 R, their selectivity, molecular mode of action and pharmacokinetic properties have been poorly characterized. Here we report the most extensive characterization of the molecular pharmacology of the most widely used CB 2 R ligands to date. In a collaborative effort between multiple academic and industry laboratories, we identify marked differences in the ability of certain agonists to activate distinct signalling pathways and to cause off-target effects. We reach a consensus that HU910, HU308 and JWH133 are the recommended selective CB 2 R agonists to study the role of CB 2 R in biological and disease processes. We believe that our unique approach would be highly suitable for the characterization of other therapeutic targets in drug discovery research.

Soethoudt, M., Grether, U., Fingerle, J., Grim, T.w., Fezza, F., De Petrocellis, L., et al. (2017). Cannabinoid CB2 receptor ligand profiling reveals biased signalling and off-target activity. NATURE COMMUNICATIONS, 8(1), 13958 [10.1038/ncomms13958].

Cannabinoid CB2 receptor ligand profiling reveals biased signalling and off-target activity

Fezza F.;Mastrangelo N.;Maccarrone M.;
2017-01-01

Abstract

The cannabinoid CB 2 receptor (CB 2 R) represents a promising therapeutic target for various forms of tissue injury and inflammatory diseases. Although numerous compounds have been developed and widely used to target CB 2 R, their selectivity, molecular mode of action and pharmacokinetic properties have been poorly characterized. Here we report the most extensive characterization of the molecular pharmacology of the most widely used CB 2 R ligands to date. In a collaborative effort between multiple academic and industry laboratories, we identify marked differences in the ability of certain agonists to activate distinct signalling pathways and to cause off-target effects. We reach a consensus that HU910, HU308 and JWH133 are the recommended selective CB 2 R agonists to study the role of CB 2 R in biological and disease processes. We believe that our unique approach would be highly suitable for the characterization of other therapeutic targets in drug discovery research.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10
English
Animals; Bridged Bicyclo Compounds; CHO Cells; Cannabinoid Receptor Agonists; Cannabinoids; Cell Line, Tumor; Cricetulus; Gene Expression; HEK293 Cells; High-Throughput Screening Assays; Humans; Keratinocytes; Kinetics; Ligands; Macrophages; Mice; Neurons; Protein Binding; Receptor, Cannabinoid, CB1; Receptor, Cannabinoid, CB2; Signal Transduction
http://www.nature.com/ncomms/index.html
Soethoudt, M., Grether, U., Fingerle, J., Grim, T.w., Fezza, F., De Petrocellis, L., et al. (2017). Cannabinoid CB2 receptor ligand profiling reveals biased signalling and off-target activity. NATURE COMMUNICATIONS, 8(1), 13958 [10.1038/ncomms13958].
Soethoudt, M; Grether, U; Fingerle, J; Grim, Tw; Fezza, F; De Petrocellis, L; Ullmer, C; Rothenhausler, B; Perret, C; Van Gils, N; Finlay, D; Macdonald, C; Chicca, A; Gens, Md; Stuart, J; De Vries, H; Mastrangelo, N; Xia, L; Alachouzos, G; Baggelaar, Mp; Martella, A; Mock, Ed; Deng, H; Heitman, Lh; Connor, M; Di Marzo, V; Gertsch, J; Lichtman, Ah; Maccarrone, M; Pacher, P; Glass, M; Van Der Stelt, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/227305
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