This study, using an in vitro ovary culture model, investigates the mechanisms through which di(2-ethylhexyl)phthalate (DEHP) impairs germ cell cyst breakdown and primordial follicle assembly. The results indicate the latter effects exerted by 10 or 100 µmol/L DEHP in cultured newborn ovaries were associated with increased levels of reactive oxygen species (ROS) and apoptosis. Based on a transcriptome analysis, we found the expression of the oxidative stress–related gene Xdh (xanthine dehydrogenase) was significantly upregulated in DEHP-cultured ovaries. Two treatments, namely Xdh RNAi or the addition of melatonin to the ovary culture, inhibited the increase in Xdh expression and ROS levels caused by DEHP and, at the same time, reduced apoptosis and the impairment of primordial follicle assembly in the treated ovaries. Together, the results identify Xdh gene as one of the major targets of DEHP in newborn ovaries and that the consequent increased level of ROS is possibly responsible for the increment of apoptosis and primordial follicle assembly impairment. At the same time, they highlight that melatonin alleviates the effects of DEHP as with other endocrine-disrupting compounds on the ovary.

Liu, J.-., Li, L., Yan, H.-., Zhang, T., Zhang, P., Sun, Z.-., et al. (2019). Identification of oxidative stress–related Xdh gene as a di(2-ethylhexyl)phthalate (DEHP) target and the use of melatonin to alleviate the DEHP-induced impairments in newborn mouse ovaries. JOURNAL OF PINEAL RESEARCH, 67(1), e12577 [10.1111/jpi.12577].

Identification of oxidative stress–related Xdh gene as a di(2-ethylhexyl)phthalate (DEHP) target and the use of melatonin to alleviate the DEHP-induced impairments in newborn mouse ovaries

De Felici M.;
2019-01-01

Abstract

This study, using an in vitro ovary culture model, investigates the mechanisms through which di(2-ethylhexyl)phthalate (DEHP) impairs germ cell cyst breakdown and primordial follicle assembly. The results indicate the latter effects exerted by 10 or 100 µmol/L DEHP in cultured newborn ovaries were associated with increased levels of reactive oxygen species (ROS) and apoptosis. Based on a transcriptome analysis, we found the expression of the oxidative stress–related gene Xdh (xanthine dehydrogenase) was significantly upregulated in DEHP-cultured ovaries. Two treatments, namely Xdh RNAi or the addition of melatonin to the ovary culture, inhibited the increase in Xdh expression and ROS levels caused by DEHP and, at the same time, reduced apoptosis and the impairment of primordial follicle assembly in the treated ovaries. Together, the results identify Xdh gene as one of the major targets of DEHP in newborn ovaries and that the consequent increased level of ROS is possibly responsible for the increment of apoptosis and primordial follicle assembly impairment. At the same time, they highlight that melatonin alleviates the effects of DEHP as with other endocrine-disrupting compounds on the ovary.
2019
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
Settore MED/40 - GINECOLOGIA E OSTETRICIA
English
Con Impact Factor ISI
apoptosis; DEHP; melatonin; primordial follicle assembly; Xdh; Animals; Animals, Newborn; Apoptosis; Diethylhexyl Phthalate; Female; Gene Expression Regulation, Enzymologic; Melatonin; Mice; Ovary; Reactive Oxygen Species; Up-Regulation; Xanthine Dehydrogenase
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1600-079X
Liu, J.-., Li, L., Yan, H.-., Zhang, T., Zhang, P., Sun, Z.-., et al. (2019). Identification of oxidative stress–related Xdh gene as a di(2-ethylhexyl)phthalate (DEHP) target and the use of melatonin to alleviate the DEHP-induced impairments in newborn mouse ovaries. JOURNAL OF PINEAL RESEARCH, 67(1), e12577 [10.1111/jpi.12577].
Liu, J-; Li, L; Yan, H-; Zhang, T; Zhang, P; Sun, Z-; De Felici, M; Reiter, Rj; Shen, W
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/227226
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