There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-α signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2'-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2'-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-α-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.

Aquilano, K., Ceci, V., Gismondi, A., De Stefano, S., Iacovelli, F., Faraonio, R., et al. (2019). Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts. COMMUNICATIONS BIOLOGY, 2(1), 317 [10.1038/s42003-019-0563-7].

Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts

Aquilano K.;Gismondi A.;Di Marco G.;Poerio N.;Minutolo A.;Fraziano M.;Potesta M.;Bernardini R.;Mattei M.;Falconi M.;Montesano C.;Rufini S.;Canini A.;Lettieri-Barbato D.
2019-01-01

Abstract

There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-α signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2'-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2'-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-α-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.
2019
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/09 - FISIOLOGIA
Settore BIO/10 - BIOCHIMICA
English
Disease model; Metabolism; Molecular medicine
Aquilano, K., Ceci, V., Gismondi, A., De Stefano, S., Iacovelli, F., Faraonio, R., et al. (2019). Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts. COMMUNICATIONS BIOLOGY, 2(1), 317 [10.1038/s42003-019-0563-7].
Aquilano, K; Ceci, V; Gismondi, A; De Stefano, S; Iacovelli, F; Faraonio, R; Di Marco, G; Poerio, N; Minutolo, A; Minopoli, G; Marcone, A; Fraziano, M; Tortolici, F; Sennato, S; Casciardi, S; Potesta, M; Bernardini, R; Mattei, M; Falconi, M; Montesano, C; Rufini, S; Canini, A; Lettieri-Barbato, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/225426
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