The influence of conformational dynamics on the self-assembly process of a conformationally constrained analogue of the natural antimicrobial peptide Trichogin GA IV was analysed by spectroscopic methods, microscopy imaging at nanometre resolution, and molecular dynamics simulations. The formation of peptide films at the air/water interface and their deposition on a graphite or a mica substrate were investigated. A combination of experimental evidence with molecular dynamics simulation was used to demonstrate that only the fully developed helical structure of the analogue promotes formation of ordered aggregates that nucleate the growth of micrometric rods, which give rise to homogenous coating over wide regions of the hydrophilic mica. This work proves the influence of helix flexibility on peptide self-organization and orientation on surfaces, key steps in the design of bioinspired organic/inorganic hybrid materials.

De Zotti, M., Corvi, G., Gatto, E., Di Napoli, B., Mazzuca, C., Palleschi, A., et al. (2019). Controlling the Formation of Peptide Films: Fully Developed Helical Peptides are Required to Obtain a Homogenous Coating over a Large Area. CHEMPLUSCHEM, 84(11), 1688-1696 [10.1002/cplu.201900456].

Controlling the Formation of Peptide Films: Fully Developed Helical Peptides are Required to Obtain a Homogenous Coating over a Large Area

Gatto E.;Di Napoli B.;Mazzuca C.;Palleschi A.;Venanzi M.
2019

Abstract

The influence of conformational dynamics on the self-assembly process of a conformationally constrained analogue of the natural antimicrobial peptide Trichogin GA IV was analysed by spectroscopic methods, microscopy imaging at nanometre resolution, and molecular dynamics simulations. The formation of peptide films at the air/water interface and their deposition on a graphite or a mica substrate were investigated. A combination of experimental evidence with molecular dynamics simulation was used to demonstrate that only the fully developed helical structure of the analogue promotes formation of ordered aggregates that nucleate the growth of micrometric rods, which give rise to homogenous coating over wide regions of the hydrophilic mica. This work proves the influence of helix flexibility on peptide self-organization and orientation on surfaces, key steps in the design of bioinspired organic/inorganic hybrid materials.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02 - Chimica Fisica
Settore CHIM/06 - Chimica Organica
English
helix formation; Langmuir-Blodgett technique; molecular dynamics simulations; peptide films; peptide microstructures
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2192-6506
De Zotti, M., Corvi, G., Gatto, E., Di Napoli, B., Mazzuca, C., Palleschi, A., et al. (2019). Controlling the Formation of Peptide Films: Fully Developed Helical Peptides are Required to Obtain a Homogenous Coating over a Large Area. CHEMPLUSCHEM, 84(11), 1688-1696 [10.1002/cplu.201900456].
De Zotti, M; Corvi, G; Gatto, E; Di Napoli, B; Mazzuca, C; Palleschi, A; Placidi, E; Biondi, B; Crisma, M; Formaggio, F; Toniolo, C; Venanzi, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/225423
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