The survival limits of the desert cyanobacterium Chroococcidiopsis were challenged by rewetting dried biofilms and dried biofilms exposed to 1.5 x 10(3) kJ/m(2) of a Mars-like UV, after 7 years of air-dried storage. PCR-stop assays revealed the presence of DNA lesions in dried biofilms and an increased accumulation in dried-UV-irradiated biofilms. Different types and/or amounts of DNA lesions were highlighted by a different expression of uvrA, uvrB, uvrC, phrA, and uvsE genes in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, after rehydration for 30 and 60 min. The up-regulation in dried-rewetted biofilms of uvsE gene encoding an UV damage endonuclease, suggested that UV-damage DNA repair contributed to the repair of desiccation-induced damage. While the phrA gene encoding a photolyase was up-regulated only in dried-UV-irradiated-rewetted biofilms. Nucleotide excision repair genes were over-expressed in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, with uvrC gene showing the highest increase in dried-UV-irradiated-rewetted biofilms. Dried biofilms preserved intact mRNAs (at least of the investigated genes) and 16S ribosomal RNA that the persistence of the ribosome machinery and mRNAs might have played a key role in the early phase recovery. Results have implications for the search of extra-terrestrial life by contributing to the definition of habitability of astrobiologically relevant targets such as Mars or planets orbiting around other stars.

Mosca, C., Rothschild, L., Napoli, A., Ferre, F., Pietrosanto, M., Fagliarone, C., et al. (2019). Over-expression of UV-damage DNA repair genes and ribonucleic acid persistence contribute to the resilience of dried biofilms of the desert cyanobacetrium Chroococcidiopsis exposed to Mars-Like UV flux and long-term desiccation. FRONTIERS IN MICROBIOLOGY, 10, 2312 [10.3389/fmicb.2019.02312].

Over-expression of UV-damage DNA repair genes and ribonucleic acid persistence contribute to the resilience of dried biofilms of the desert cyanobacetrium Chroococcidiopsis exposed to Mars-Like UV flux and long-term desiccation

Pietrosanto, M;Fagliarone, C;Baque, M;Billi, D
2019

Abstract

The survival limits of the desert cyanobacterium Chroococcidiopsis were challenged by rewetting dried biofilms and dried biofilms exposed to 1.5 x 10(3) kJ/m(2) of a Mars-like UV, after 7 years of air-dried storage. PCR-stop assays revealed the presence of DNA lesions in dried biofilms and an increased accumulation in dried-UV-irradiated biofilms. Different types and/or amounts of DNA lesions were highlighted by a different expression of uvrA, uvrB, uvrC, phrA, and uvsE genes in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, after rehydration for 30 and 60 min. The up-regulation in dried-rewetted biofilms of uvsE gene encoding an UV damage endonuclease, suggested that UV-damage DNA repair contributed to the repair of desiccation-induced damage. While the phrA gene encoding a photolyase was up-regulated only in dried-UV-irradiated-rewetted biofilms. Nucleotide excision repair genes were over-expressed in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, with uvrC gene showing the highest increase in dried-UV-irradiated-rewetted biofilms. Dried biofilms preserved intact mRNAs (at least of the investigated genes) and 16S ribosomal RNA that the persistence of the ribosome machinery and mRNAs might have played a key role in the early phase recovery. Results have implications for the search of extra-terrestrial life by contributing to the definition of habitability of astrobiologically relevant targets such as Mars or planets orbiting around other stars.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/01
English
DNA repair; Mars UV simulation; anhydrobiosis; desert cyanobacteria; habitability and astrobiology
Mosca, C., Rothschild, L., Napoli, A., Ferre, F., Pietrosanto, M., Fagliarone, C., et al. (2019). Over-expression of UV-damage DNA repair genes and ribonucleic acid persistence contribute to the resilience of dried biofilms of the desert cyanobacetrium Chroococcidiopsis exposed to Mars-Like UV flux and long-term desiccation. FRONTIERS IN MICROBIOLOGY, 10, 2312 [10.3389/fmicb.2019.02312].
Mosca, C; Rothschild, L; Napoli, A; Ferre, F; Pietrosanto, M; Fagliarone, C; Baque, M; Rabbow, E; Rettberg, P; Billi, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/225221
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