Objectives: The aim of this study is to characterize a new bacteriophage able to infect Enterococcus faecalis, and to evaluate its ability to disrupt biofilm. Methods: The vB_EfaH_EF1TV (EF1TV) host-range was determined by spot test and efficiency of plating using a collection of 15 E. faecalis clinical strains. The phage genome was sequenced with a next generation sequencing approach. Anti-biofilm activity was tested by crystal violet method and confocal laser scanning microscopy. Phage-resistant mutants were selected and sequenced to investigate receptors exploited by phage for infection. Results: EF1TV is a newly discovered E. faecalis phage which belongs to the Herelleviridae family. EF1TV, whose genome is 98% identical to φEF24C, is characterized by a linear dsDNA genome of 143,507 bp with direct terminal repeats of 1,911 bp. The phage is able to infect E. faecalis and shows also the ability to degrade biofilm produced by strains of this species. The results were confirmed by confocal laser scanning microscopy analyzing the biofilm reduction in the same optical field before and after phage infection. Conclusions: The EF1TV phage shows promising features such as an obligatory lytic nature, an anti-biofilm activity and the absence of integration-related proteins, antibiotic resistance determinants and virulence factors, and therefore could be a promising tool for therapeutic applications.

D'Andrea, M.m., Frezza, D., Romano, E., Marmo, P., Henrici De Angelis, L., Perini, N., et al. (2019). The lytic bacteriophage vB_EfaH_EF1TV, a new member of the Herelleviridae family, disrupts biofilm produced by Enterococcus faecalis clinical strains. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE [10.1016/j.jgar.2019.10.019].

The lytic bacteriophage vB_EfaH_EF1TV, a new member of the Herelleviridae family, disrupts biofilm produced by Enterococcus faecalis clinical strains

Marco Maria D’Andrea;Domenico Frezza;Elena Romano;Pasquale Marmo;Nicoletta Perini;Maria Cristina Thaller;Gustavo Di Lallo
2019-10-31

Abstract

Objectives: The aim of this study is to characterize a new bacteriophage able to infect Enterococcus faecalis, and to evaluate its ability to disrupt biofilm. Methods: The vB_EfaH_EF1TV (EF1TV) host-range was determined by spot test and efficiency of plating using a collection of 15 E. faecalis clinical strains. The phage genome was sequenced with a next generation sequencing approach. Anti-biofilm activity was tested by crystal violet method and confocal laser scanning microscopy. Phage-resistant mutants were selected and sequenced to investigate receptors exploited by phage for infection. Results: EF1TV is a newly discovered E. faecalis phage which belongs to the Herelleviridae family. EF1TV, whose genome is 98% identical to φEF24C, is characterized by a linear dsDNA genome of 143,507 bp with direct terminal repeats of 1,911 bp. The phage is able to infect E. faecalis and shows also the ability to degrade biofilm produced by strains of this species. The results were confirmed by confocal laser scanning microscopy analyzing the biofilm reduction in the same optical field before and after phage infection. Conclusions: The EF1TV phage shows promising features such as an obligatory lytic nature, an anti-biofilm activity and the absence of integration-related proteins, antibiotic resistance determinants and virulence factors, and therefore could be a promising tool for therapeutic applications.
31-ott-2019
Online ahead of print
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/19 - MICROBIOLOGIA GENERALE
English
Con Impact Factor ISI
Phage therapy; biofilm degradation; Herelleviridae family; SPO1-like phage; bacteriophage resistance.
https://doi.org/10.1016/j.jgar.2019.10.019
D'Andrea, M.m., Frezza, D., Romano, E., Marmo, P., Henrici De Angelis, L., Perini, N., et al. (2019). The lytic bacteriophage vB_EfaH_EF1TV, a new member of the Herelleviridae family, disrupts biofilm produced by Enterococcus faecalis clinical strains. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE [10.1016/j.jgar.2019.10.019].
D'Andrea, Mm; Frezza, D; Romano, E; Marmo, P; Henrici De Angelis, L; Perini, N; Thaller, Mc; DI LALLO, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/224856
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