The aim of the present work was to investigate possible effects of culture in the Rotary Cell Culture System (RCCS) bioreactor simulating microgravity conditions on early stages of embryo formation using embryoid bodies (EBs) as an experimental model. Expression of five genes was analyzed (Gata-1, Brachyury, Nanog, Oct-4, Hnf-4) in such conditions crucial for cell linage differentiation events occurring in the preimplantation embryo. EBs derived from mice embryonic stem cells (ESCs) were encapsulated in alginate to prevent they aggregation in RCCS. Results indicate that microgravity seems at least at longer time culture to counteract, the increased EB growth observed in to alginate beads
Nowacki, D., DE FELICI, M. (2011). Effect of culture in simulatred microgravity on the development of the embryoid bodies. In Podbielska H, Trziszka T (a cura di), Nutraceutics, biomedical and physiotherapeutic methods for prevention of civilization-related diseases (pp. 181-192). Sociietas Humboldtiana Polonorum.
Effect of culture in simulatred microgravity on the development of the embryoid bodies
DE FELICI, MASSIMO
2011-01-01
Abstract
The aim of the present work was to investigate possible effects of culture in the Rotary Cell Culture System (RCCS) bioreactor simulating microgravity conditions on early stages of embryo formation using embryoid bodies (EBs) as an experimental model. Expression of five genes was analyzed (Gata-1, Brachyury, Nanog, Oct-4, Hnf-4) in such conditions crucial for cell linage differentiation events occurring in the preimplantation embryo. EBs derived from mice embryonic stem cells (ESCs) were encapsulated in alginate to prevent they aggregation in RCCS. Results indicate that microgravity seems at least at longer time culture to counteract, the increased EB growth observed in to alginate beadsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
