In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soaked with an agar thin film and placed in a glass optical measurement cell, obtaining a totally environmental-friendly device. Nanoencapsulated-atrazine was detected by following the variable fluorescence (1-VJ) parameter, which decreased inversely proportional to the herbicide concentrations, in a range between 0.5 and 200 nM, indicating a linear relationship in the measured dose-response curves and a detection limit of 4 pM. Interference studies resulted in a very slight interference in presence of 2 ppm copper and 10 ppb arsenic at safety limits, as well as a slight matrix effect and a satisfactory recovery value of 96 ± 5% for 75 nM nanoencapsulated-atrazine in tap water. Stability studies were also performed obtaining a good storage stability up to 3 weeks. Results demonstrated the suitability of the proposed paper-based optical biosensor as a valid support in smart agriculture for on site, environmental friendly, cost effective and sensitive nanoencapsulated-atrazine analysis.

Scognamiglio, V., Antonacci, A., Arduini, F., Moscone, D., Campos, E., Fraceto, L.f., et al. (2019). An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection. JOURNAL OF HAZARDOUS MATERIALS, 373, 483-492 [10.1016/j.jhazmat.2019.03.082].

An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection

Arduini F.;Moscone D.;Palleschi G.
2019-07-01

Abstract

In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soaked with an agar thin film and placed in a glass optical measurement cell, obtaining a totally environmental-friendly device. Nanoencapsulated-atrazine was detected by following the variable fluorescence (1-VJ) parameter, which decreased inversely proportional to the herbicide concentrations, in a range between 0.5 and 200 nM, indicating a linear relationship in the measured dose-response curves and a detection limit of 4 pM. Interference studies resulted in a very slight interference in presence of 2 ppm copper and 10 ppb arsenic at safety limits, as well as a slight matrix effect and a satisfactory recovery value of 96 ± 5% for 75 nM nanoencapsulated-atrazine in tap water. Stability studies were also performed obtaining a good storage stability up to 3 weeks. Results demonstrated the suitability of the proposed paper-based optical biosensor as a valid support in smart agriculture for on site, environmental friendly, cost effective and sensitive nanoencapsulated-atrazine analysis.
lug-2019
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Paper-based algal biosensorChlamydomonas reinhardtiiChlorophyll fluorescenceNanoencapsulated-atrazineSustainable agriculture
Scognamiglio, V., Antonacci, A., Arduini, F., Moscone, D., Campos, E., Fraceto, L.f., et al. (2019). An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection. JOURNAL OF HAZARDOUS MATERIALS, 373, 483-492 [10.1016/j.jhazmat.2019.03.082].
Scognamiglio, V; Antonacci, A; Arduini, F; Moscone, D; Campos, Evr; Fraceto, Lf; Palleschi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/215747
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