Vermamoeba vermiformis represents one of the most common free-living amoebae identified in worldwide environmental surveys. We analyzed 56 water samples with varying characteristics, including temperature and the particular settings in which humans may be exposed to water, plus one corneal scraping from a keratitis patient, with the following aims: (i) to investigate the presence of V. vermiformis; (ii) to identify the isolate subtypes; (iii) to place the Italian isolates in the broader picture of the genetic diversity within V. vermiformis. Twenty-two isolates were identified upon culturing and sequencing of > 600 bp in the 18S ribosomal RNA (rRNA) gene sequence, bringing to 27 the number of sequences recovered from Italian sources. By adding deposited sequences, we assembled a dataset of 74 isolates. Three of our isolates were characterized by allelic code 7-5-1-1, never reported before, and two showed 100% identity with an uncultured eukaryote and carried the 719T>C variant. We show that the variable segments E5, E3, F, and G convey most of the information on diversity, enabling the clustering of the isolates in a replicable fashion. The presence of different strains in natural thermal waters and in distribution systems indicated heterogeneity of the amoebic populations. Also, ours and the only other sequence from human infection were mapped in different clades. Overall, we enlarged the repertoire of single nucleotide and indel variants and the list of allelic codes, proceeding one step further in the description of the diversity within the genus.

Montalbano Di Filippo, M., Berrilli, F., Di Cave, D., Novelletto, A. (2019). Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus. PARASITOLOGY RESEARCH, 118(6), 1751-1759 [10.1007/s00436-019-06294-x].

Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus

Montalbano Di Filippo, M;Berrilli, F;Di Cave, D;Novelletto, A
2019

Abstract

Vermamoeba vermiformis represents one of the most common free-living amoebae identified in worldwide environmental surveys. We analyzed 56 water samples with varying characteristics, including temperature and the particular settings in which humans may be exposed to water, plus one corneal scraping from a keratitis patient, with the following aims: (i) to investigate the presence of V. vermiformis; (ii) to identify the isolate subtypes; (iii) to place the Italian isolates in the broader picture of the genetic diversity within V. vermiformis. Twenty-two isolates were identified upon culturing and sequencing of > 600 bp in the 18S ribosomal RNA (rRNA) gene sequence, bringing to 27 the number of sequences recovered from Italian sources. By adding deposited sequences, we assembled a dataset of 74 isolates. Three of our isolates were characterized by allelic code 7-5-1-1, never reported before, and two showed 100% identity with an uncultured eukaryote and carried the 719T>C variant. We show that the variable segments E5, E3, F, and G convey most of the information on diversity, enabling the clustering of the isolates in a replicable fashion. The presence of different strains in natural thermal waters and in distribution systems indicated heterogeneity of the amoebic populations. Also, ours and the only other sequence from human infection were mapped in different clades. Overall, we enlarged the repertoire of single nucleotide and indel variants and the list of allelic codes, proceeding one step further in the description of the diversity within the genus.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
English
18S rRNA; Allelic variants; Amoebozoa; Dynamic homology; Vermamoeba vermiformis
Montalbano Di Filippo, M., Berrilli, F., Di Cave, D., Novelletto, A. (2019). Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus. PARASITOLOGY RESEARCH, 118(6), 1751-1759 [10.1007/s00436-019-06294-x].
Montalbano Di Filippo, M; Berrilli, F; Di Cave, D; Novelletto, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/212786
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