A factor produced by several strains of Escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on CHO, Vero, and HeLa cells. The same strains were found to have hemolytic activity on sheep erythrocytes. The toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from Vero toxin. The main effect induced by the isolated factor on cultured cells was the formation of large multinucleated cells. The partial purification achieved suggests that the same factor (most likely a protein with a molecular weight of 70,000 to 80,000) is responsible for toxic and cell-altering activities, whereas a different molecular species is responsible for hemolytic activity.

Caprioli, A., Falbo, V., Roda, L.g., Ruggeri, F.m., Zona, C. (1983). Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. INFECTION AND IMMUNITY, 39(3), 1300-1306.

Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations

Zona, C
Investigation
1983-03-01

Abstract

A factor produced by several strains of Escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on CHO, Vero, and HeLa cells. The same strains were found to have hemolytic activity on sheep erythrocytes. The toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from Vero toxin. The main effect induced by the isolated factor on cultured cells was the formation of large multinucleated cells. The partial purification achieved suggests that the same factor (most likely a protein with a molecular weight of 70,000 to 80,000) is responsible for toxic and cell-altering activities, whereas a different molecular species is responsible for hemolytic activity.
mar-1983
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/09 - FISIOLOGIA
English
Animals; Bacterial Toxins; Cell Line; Cell Nucleus; Cercopithecus aethiops; Cricetinae; Cytotoxins; Escherichia coli; Female; Guinea Pigs; HeLa Cells; Hemolysis; Hot Temperature; Humans; Hydrogen-Ion Concentration; Necrosis; Ovary; Rabbits; Sheep
Caprioli, A., Falbo, V., Roda, L.g., Ruggeri, F.m., Zona, C. (1983). Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. INFECTION AND IMMUNITY, 39(3), 1300-1306.
Caprioli, A; Falbo, V; Roda, Lg; Ruggeri, Fm; Zona, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/211435
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