Psoriasis is a diffuse chronic skin disorder characterized from accelerated epidermal turnover and inflammatory cell infiltrate. Retinoids influence keratinocyte proliferation and differentiation as well as inflammatory response. Cellular retinol binding protein (CRBPI) regulates intracellular vitamin A bioavailability and contributes to maintain skin homeostasis. The aim of present study was to investigate the expression of CRBPI and its role in the pathogenesis of skin psoriasis. Immunohistochemistry revealed more diffuse and increased CRBPI expression in all epidermal layers of human psoriatic lesions except in the stratum corneum. An imiquimod-induced psoriatic-like model documented the increase of skin lesional area and severity index score as well as of the severity of microscopic features as parakeratosis, papillomatosis and spongiosis in CRBPI-knockout compared to wild-type mice, associated to the increased keratinocyte CK17 and Ki-67 expression and the reduction of CK1, CRABPII and RXRα. Gene array of imiquimod-induced psoriatic skin documented the greater up-regulation of EGF/PDGF-related genes and down-regulation of EGR1 and pro-inflammatory IL-related genes in CRBPI-knockout compared to wild-type mice. Finally, CRBPI transfection in HaCaT cells increased AKT and NF-κB-related genes and proteins and down-regulated IL-2, IL-6 and IL-8 pro-inflammatory signalling. Although not recognized as a psoriatic susceptibility gene in our cohort of patients, the present data strongly supported the potential role of CRBPI to sustain keratinocyte proliferation and differentiation and to counteract pro-inflammatory genes expression in psoriatic lesions.

Costanza, G., Doldo, E., Ferlosio, A., Tarquini, C., Passeri, D., Cascella, R., et al. (2018). Expression and potential role of cellular retinol binding protein I in psoriasis. ONCOTARGET, 9(95), 36736-36749 [10.18632/oncotarget.26314].

Expression and potential role of cellular retinol binding protein I in psoriasis

Doldo, Elena;Ferlosio, Amedeo;Tarquini, Chiara;Passeri, Daniela;Cascella, Raffaella;Di Stefani, Alessandro;Agostinelli, Sara;Giardina, Emiliano;Campione, Elena;Bianchi, Luca;Orlandi, Augusto
2018-12-04

Abstract

Psoriasis is a diffuse chronic skin disorder characterized from accelerated epidermal turnover and inflammatory cell infiltrate. Retinoids influence keratinocyte proliferation and differentiation as well as inflammatory response. Cellular retinol binding protein (CRBPI) regulates intracellular vitamin A bioavailability and contributes to maintain skin homeostasis. The aim of present study was to investigate the expression of CRBPI and its role in the pathogenesis of skin psoriasis. Immunohistochemistry revealed more diffuse and increased CRBPI expression in all epidermal layers of human psoriatic lesions except in the stratum corneum. An imiquimod-induced psoriatic-like model documented the increase of skin lesional area and severity index score as well as of the severity of microscopic features as parakeratosis, papillomatosis and spongiosis in CRBPI-knockout compared to wild-type mice, associated to the increased keratinocyte CK17 and Ki-67 expression and the reduction of CK1, CRABPII and RXRα. Gene array of imiquimod-induced psoriatic skin documented the greater up-regulation of EGF/PDGF-related genes and down-regulation of EGR1 and pro-inflammatory IL-related genes in CRBPI-knockout compared to wild-type mice. Finally, CRBPI transfection in HaCaT cells increased AKT and NF-κB-related genes and proteins and down-regulated IL-2, IL-6 and IL-8 pro-inflammatory signalling. Although not recognized as a psoriatic susceptibility gene in our cohort of patients, the present data strongly supported the potential role of CRBPI to sustain keratinocyte proliferation and differentiation and to counteract pro-inflammatory genes expression in psoriatic lesions.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - Anatomia Patologica
English
CRBPI; imiquimod-induced psoriasis; keratinocyte proliferation; retinoids
Costanza, G., Doldo, E., Ferlosio, A., Tarquini, C., Passeri, D., Cascella, R., et al. (2018). Expression and potential role of cellular retinol binding protein I in psoriasis. ONCOTARGET, 9(95), 36736-36749 [10.18632/oncotarget.26314].
Costanza, G; Doldo, E; Ferlosio, A; Tarquini, C; Passeri, D; Cascella, R; Bavetta, M; Di Stefani, A; Bonifati, C; Agostinelli, S; Centofanti, F; Giardina, E; Campione, E; Bianchi, L; Donati, P; Morrone, A; Orlandi, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/210720
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