The generation of the daunomycin semiquinone was studied in intact red blood cells under CO atmosphere by ESR spectroscopy. The undialyzed hemolysates and the spin broadening agent chromium oxalate quenched the ESR signal, suggesting external location of the ESR-detectable radicals and their slow diffusion inside. A constant outward flow of O2- was detected by monitoring the approach to the steady state of the ESR signal of Cu,Zn superoxide dismutase externally added to red blood cells plus daunomycin in air. This suggests a reductase on the outer side of the erythrocyte membrane as the source of daunomycin radicals.

Pedersen, J., Marcocci, L., Rossi, L., Mavelli, I., Rotilio, G. (1990). Generation of daunomycin radicals on the outer side of the erythrocyte membrane. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 168(1), 240-247.

Generation of daunomycin radicals on the outer side of the erythrocyte membrane

Pedersen JZ;Rossi L;Rotilio G
1990-04-16

Abstract

The generation of the daunomycin semiquinone was studied in intact red blood cells under CO atmosphere by ESR spectroscopy. The undialyzed hemolysates and the spin broadening agent chromium oxalate quenched the ESR signal, suggesting external location of the ESR-detectable radicals and their slow diffusion inside. A constant outward flow of O2- was detected by monitoring the approach to the steady state of the ESR signal of Cu,Zn superoxide dismutase externally added to red blood cells plus daunomycin in air. This suggests a reductase on the outer side of the erythrocyte membrane as the source of daunomycin radicals.
16-apr-1990
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10
English
Con Impact Factor ISI
Daunorubicin; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane; Free Radicals; Humans; In Vitro Techniques; Superoxides; Time Factors
Pedersen, J., Marcocci, L., Rossi, L., Mavelli, I., Rotilio, G. (1990). Generation of daunomycin radicals on the outer side of the erythrocyte membrane. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 168(1), 240-247.
Pedersen, J; Marcocci, L; Rossi, L; Mavelli, I; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/210136
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