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EnglishThe mitochondrial permeability transition pore and Bax have both been proposed to be involved in the release of pro-apoptotic factors from mitochondria in the "intrinsic" pathway of apoptosis. The permeability transition pore is widely thought to be a supramolecular complex including or interacting with Bax. Given the relevance of the permeability transition in vivo, we have verified whether Bax influences the formation and/or the properties of the Ca(2+)/P(i)-induced permeability transition by using mitochondriaisolated from isogenic human colon cancer bax(+/-) and bax(-/-) HCT116 cell lines. We used mitochondria isolated from both types of cells and from Bax(+) cells exposed to apoptotic stimuli, as well as Bax-less mitochondria into which exogenous Bax had been incorporated. All exhibited the same behavior and pharmacological profile in swelling and Ca(2+)-retention experiments. Mitochondria from a bax(-)/bak(-) cell line also underwent an analogous Ca(2+)/P(i)-inducible swelling. This similarity indicates that Bax hasno major role in regulating the Ca(2+)-induced mitochondrial permeability transition.
De Marchi, U., Campello, S., Szabo, I., Tombola, F., Martinou, J.-., & Zoratti, M. (2004). Bax does not directly participate in the Ca2+-induced permeability transition of isolated mitochondria. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 279(36), 37415-37422 [10.1074/jbc.M314093200].
| Tipologia: | Articolo su rivista | |
| Citazione: | De Marchi, U., Campello, S., Szabo, I., Tombola, F., Martinou, J.-., & Zoratti, M. (2004). Bax does not directly participate in the Ca2+-induced permeability transition of isolated mitochondria. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 279(36), 37415-37422 [10.1074/jbc.M314093200]. | |
| Lingua: | English | |
| Settore Scientifico Disciplinare: | Settore BIO/09 | |
| Revisione (peer review): | Esperti anonimi | |
| Tipo: | Articolo | |
| Rilevanza: | Rilevanza internazionale | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1074/jbc.M314093200 | |
| Stato di pubblicazione: | Pubblicato | |
| Data di pubblicazione: | 2004 | |
| Titolo: | Bax does not directly participate in the Ca2+-induced permeability transition of isolated mitochondria | |
| Autori: | ||
| Autori: | De Marchi, U; Campello, S; Szabo, I; Tombola, F; Martinou, J-; Zoratti, M | |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 5 2004 Campello JBC.pdf | N/A | Copyright dell'editore | Open Access Visualizza/Apri |
http://hdl.handle.net/2108/207041
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