23 patients (18 male and 5 female) aged 21-70 years who displayed male pattern hair loss (MPHL) in Stage 1 to Stage 5 as determined by the Norwood-Hamilton classification scale, and female pattern hair loss (FPHL) in Stage 1 to Stage 2 as determined by the Ludwig classification scale, were treated with non-activated autologous platelet-rich plasma (A-PRP). Autologous blood (55 mL) was harvested using sodium citrate as an anticoagulant. A-PRP (23 mL) was produced for all cases using a closed system according to the transfusion service protocol. Following centrifugation (260 x g for 10 min) the A-PRP was inserted in a laser light selector device, and after the centrifugation, 9 mL of A-PRP was collected. The scalp of the patients affected by androgenetic alopecia (AGA) was divided into four areas (frontal, parietal, vertex, and occipital); local anesthesia was not performed. Interfollicular A-PRP injections (0.2 mL x cm2) were performed by controlled and mechanical injections scheduled at a depth of 5 mm using a medical injector gun. Treatment sessions were performed with a 30-day interval. For each patient, three treatment sessions were performed. PRP was injected in the androgen-related areas of scalp affected by hair loss. Placebo (normal saline solution) was loaded in another syringe (10 mL) and injected on the adjacent side in a similar fashion.

Gentile, P., Garcovich, S., Scioli, M.g., Bielli, A., Orlandi, A., & Cervelli, V. (2018). Mechanical and controlled PRP injections in patients affected by androgenetic alopecia. JOURNAL OF VISUALIZED EXPERIMENTS, 2018(131) [10.3791/56406].

Mechanical and controlled PRP injections in patients affected by androgenetic alopecia

Gentile P.;Bielli A.;Orlandi A.;Cervelli V.
2018

Abstract

23 patients (18 male and 5 female) aged 21-70 years who displayed male pattern hair loss (MPHL) in Stage 1 to Stage 5 as determined by the Norwood-Hamilton classification scale, and female pattern hair loss (FPHL) in Stage 1 to Stage 2 as determined by the Ludwig classification scale, were treated with non-activated autologous platelet-rich plasma (A-PRP). Autologous blood (55 mL) was harvested using sodium citrate as an anticoagulant. A-PRP (23 mL) was produced for all cases using a closed system according to the transfusion service protocol. Following centrifugation (260 x g for 10 min) the A-PRP was inserted in a laser light selector device, and after the centrifugation, 9 mL of A-PRP was collected. The scalp of the patients affected by androgenetic alopecia (AGA) was divided into four areas (frontal, parietal, vertex, and occipital); local anesthesia was not performed. Interfollicular A-PRP injections (0.2 mL x cm2) were performed by controlled and mechanical injections scheduled at a depth of 5 mm using a medical injector gun. Treatment sessions were performed with a 30-day interval. For each patient, three treatment sessions were performed. PRP was injected in the androgen-related areas of scalp affected by hair loss. Placebo (normal saline solution) was loaded in another syringe (10 mL) and injected on the adjacent side in a similar fashion.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/19 - Chirurgia Plastica
English
Adult; Aged; Alopecia; Female; Humans; Injections; Male; Middle Aged; Platelet-Rich Plasma; Young Adult
Gentile, P., Garcovich, S., Scioli, M.g., Bielli, A., Orlandi, A., & Cervelli, V. (2018). Mechanical and controlled PRP injections in patients affected by androgenetic alopecia. JOURNAL OF VISUALIZED EXPERIMENTS, 2018(131) [10.3791/56406].
Gentile, P; Garcovich, S; Scioli, Mg; Bielli, A; Orlandi, A; Cervelli, V
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/207027
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