Functional foods include compounds with nutritional and health properties. The human diet could play a stronger role in cancer prevention. Only a few studies have described the presence of plant small RNA, in humans who were fed with plant foods, which demonstrated the ability of these molecules to modulate consumer’s genes and evidenced the existence of a plant-animal regulation. Through in silico prediction, Olea europaea small RNAs (sRs), which had been previously reported as miRNAs, were identified, each with functional homology to hsa-miR34a. According to this initial funding, we investigated the ability of oeu-sRs to regulate tumorigenesis in human cells. The transfection of these synthetic oeu-sRs reduced the protein expression of hsa-miR34a mRNA targets, increased apoptosis and decreased proliferation in different tumor cells; by contrast, no effect was observed in PBMCs from healthy donors. The introduction of oeu-small RNA in hsa-miR34a-deficient tumor cells restores its function, whereas cells with normal expression of endogenous hsa-miR34a remained unaffected. The natural oeu-small RNAs that were extracted from O. europaea drupes induce the same effects as synthetic sRs. Careful research on the small RNA sequences executed for mapping and annotation in the genome of O. europaea var. Sylvestris and var. Farga led to the hypothesis that RNA fragments with functional homology to human miRNAs could be generated from the degradation of regions of RNA transcripts. These results indicate the possibility of developing novel natural non-toxic drugs that contain active plant-derived tumor-suppressing small RNA with functional homology to hsa-miRNAs and that can support antineoplastic strategies.

Minutolo, A., Potestà, M., Gismondi, A., Pirrò, S., Cirilli, M., Gattabria, F., et al. (2018). Olea europaea small RNA with functional homology to human miR34a in cross-kingdom interaction of anti-tumoral response. SCIENTIFIC REPORTS, 8(1), 12413 [10.1038/s41598-018-30718-w].

Olea europaea small RNA with functional homology to human miR34a in cross-kingdom interaction of anti-tumoral response

Minutolo, Antonella;Potestà, Marina;Gismondi, Angelo;Pirrò, Stefano;Galgani, Andrea;Mattei, Maurizio;Canini, Antonella;Colizzi, Vittorio;Montesano, Carla
2018

Abstract

Functional foods include compounds with nutritional and health properties. The human diet could play a stronger role in cancer prevention. Only a few studies have described the presence of plant small RNA, in humans who were fed with plant foods, which demonstrated the ability of these molecules to modulate consumer’s genes and evidenced the existence of a plant-animal regulation. Through in silico prediction, Olea europaea small RNAs (sRs), which had been previously reported as miRNAs, were identified, each with functional homology to hsa-miR34a. According to this initial funding, we investigated the ability of oeu-sRs to regulate tumorigenesis in human cells. The transfection of these synthetic oeu-sRs reduced the protein expression of hsa-miR34a mRNA targets, increased apoptosis and decreased proliferation in different tumor cells; by contrast, no effect was observed in PBMCs from healthy donors. The introduction of oeu-small RNA in hsa-miR34a-deficient tumor cells restores its function, whereas cells with normal expression of endogenous hsa-miR34a remained unaffected. The natural oeu-small RNAs that were extracted from O. europaea drupes induce the same effects as synthetic sRs. Careful research on the small RNA sequences executed for mapping and annotation in the genome of O. europaea var. Sylvestris and var. Farga led to the hypothesis that RNA fragments with functional homology to human miRNAs could be generated from the degradation of regions of RNA transcripts. These results indicate the possibility of developing novel natural non-toxic drugs that contain active plant-derived tumor-suppressing small RNA with functional homology to hsa-miRNAs and that can support antineoplastic strategies.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/01
Settore MED/04 - Patologia Generale
English
The study was supported by STARBIOS2 European Union’s Horizon 2020 research and innovation program under grant agreement No. 709517 oriented to promote Responsible Research and Innovation in biosciences,
www.nature.com/srep/index.html
Minutolo, A., Potestà, M., Gismondi, A., Pirrò, S., Cirilli, M., Gattabria, F., et al. (2018). Olea europaea small RNA with functional homology to human miR34a in cross-kingdom interaction of anti-tumoral response. SCIENTIFIC REPORTS, 8(1), 12413 [10.1038/s41598-018-30718-w].
Minutolo, A; Potestà, M; Gismondi, A; Pirrò, S; Cirilli, M; Gattabria, F; Galgani, A; Sessa, L; Mattei, M; Canini, A; Muleo, R; Colizzi, V; Montesano, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/202755
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