Electroanalysis is one of the disciplines which majorly gained from the discovery of nano/micromaterials fascinating and unique properties. These new-dimensional entities, with their versatility, easy manipulation, and chemical- physical properties, greatly improve the electroanalytical performances. Their remarkable sensitivity and costeffectiveness represent the most valuable characteristics. During this thesis, a wide range of nano/microsized materials have been synthesized, characterized, and combined with screen-printed electrodes (SPEs) , for creating ad hoc platforms. Prussian Blue (PB) has been finely nano-tailored and, by controlling the experimental conditions the analytical properties of this compound were tuned to detect hydrogen peroxide with high sensitivity and selectivity. Further integration with a microfabricated sample cell, employing just one type of the PB nanoparticle-modified SPE, has allowed the biosensing of cholesterol in serum using the enzyme cholesterol oxidase. Subsequently, a "poorly noble" but highly interesting carbonaceous nanomaterial, better known as Carbon Black (CB), has been successfully employed, in combination with gold nanoparticles (AuNPs), to effectively enhance its sensing properties and the electrocatalysis. CB-AuNPs nanocomposite-modified SPEs have been electrochemically and morphologically investigated, highlighting a synergic action of the two nanosized materials for the detection of different species such as arsenic, glucose, hydroquinone and ascorbic acid. Simple and inexpensive routes to fabricate stand-alone built-in platforms have been also evaluated in order to develop user-friendly electroanalytical devices, joining innovative nano/micromaterials to smart manufacturing strategies. Novel synthetic microengines have been rationally designed and employed. These have shown a surprising dual action with solution mixing and control of the reaction parameters, hydrolyzing paraoxon and sensing the non hazardous by-product, namely pnitrophenol, by means of printed carbon electrodes. Also, paper has been used as substrate to fabricate a general reagent-free electroanalytical platform. By following three easy steps, consisting of wax patterning, paper impregnation, and electrode screen-printing, filter paper provides an effective platform to sense, in standard and real samples. Phosphate has been chosen as model analyte and CBmodified SPE has provided a satisfactory analytical performance.

(2015). Nano/Micromaterial-driven electroanalysis enhancement: facile approaches to improve (bio)sensing.

Nano/Micromaterial-driven electroanalysis enhancement: facile approaches to improve (bio)sensing

CINTI, STEFANO
2015-01-01

Abstract

Electroanalysis is one of the disciplines which majorly gained from the discovery of nano/micromaterials fascinating and unique properties. These new-dimensional entities, with their versatility, easy manipulation, and chemical- physical properties, greatly improve the electroanalytical performances. Their remarkable sensitivity and costeffectiveness represent the most valuable characteristics. During this thesis, a wide range of nano/microsized materials have been synthesized, characterized, and combined with screen-printed electrodes (SPEs) , for creating ad hoc platforms. Prussian Blue (PB) has been finely nano-tailored and, by controlling the experimental conditions the analytical properties of this compound were tuned to detect hydrogen peroxide with high sensitivity and selectivity. Further integration with a microfabricated sample cell, employing just one type of the PB nanoparticle-modified SPE, has allowed the biosensing of cholesterol in serum using the enzyme cholesterol oxidase. Subsequently, a "poorly noble" but highly interesting carbonaceous nanomaterial, better known as Carbon Black (CB), has been successfully employed, in combination with gold nanoparticles (AuNPs), to effectively enhance its sensing properties and the electrocatalysis. CB-AuNPs nanocomposite-modified SPEs have been electrochemically and morphologically investigated, highlighting a synergic action of the two nanosized materials for the detection of different species such as arsenic, glucose, hydroquinone and ascorbic acid. Simple and inexpensive routes to fabricate stand-alone built-in platforms have been also evaluated in order to develop user-friendly electroanalytical devices, joining innovative nano/micromaterials to smart manufacturing strategies. Novel synthetic microengines have been rationally designed and employed. These have shown a surprising dual action with solution mixing and control of the reaction parameters, hydrolyzing paraoxon and sensing the non hazardous by-product, namely pnitrophenol, by means of printed carbon electrodes. Also, paper has been used as substrate to fabricate a general reagent-free electroanalytical platform. By following three easy steps, consisting of wax patterning, paper impregnation, and electrode screen-printing, filter paper provides an effective platform to sense, in standard and real samples. Phosphate has been chosen as model analyte and CBmodified SPE has provided a satisfactory analytical performance.
2015
2014/2015
Scienze chimiche
28.
Settore CHIM/03 - CHIMICA GENERALE E INORGANICA
English
Tesi di dottorato
(2015). Nano/Micromaterial-driven electroanalysis enhancement: facile approaches to improve (bio)sensing.
File in questo prodotto:
File Dimensione Formato  
PhD thesis_Stefano Cinti_Final edit.pdf

solo utenti autorizzati

Licenza: Non specificato
Dimensione 3.78 MB
Formato Adobe PDF
3.78 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/201901
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact