This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.

Alvarez-Cubero, M., Santiago, O., Martínez-Labarga, C., Martínez-García, B., Marrero- Díaz, R., Rubio-Roldan, A., et al. (2018). METHODOLOGY FOR Y CHROMOSOME CAPTURE AND ANALYSIS: A complete genome sequence of Y chromosome using Flow Cytometry Capture, Laser Capture Microdissection and Magnetic Streptavidin-Bead Capture. SCIENTIFIC REPORTS, 8(1), 9436 [10.1038/s41598-018-27819-x].

METHODOLOGY FOR Y CHROMOSOME CAPTURE AND ANALYSIS: A complete genome sequence of Y chromosome using Flow Cytometry Capture, Laser Capture Microdissection and Magnetic Streptavidin-Bead Capture

Martínez-Labarga C.;
2018-01-01

Abstract

This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.
2018
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/18 - GENETICA
English
Con Impact Factor ISI
Alvarez-Cubero, M., Santiago, O., Martínez-Labarga, C., Martínez-García, B., Marrero- Díaz, R., Rubio-Roldan, A., et al. (2018). METHODOLOGY FOR Y CHROMOSOME CAPTURE AND ANALYSIS: A complete genome sequence of Y chromosome using Flow Cytometry Capture, Laser Capture Microdissection and Magnetic Streptavidin-Bead Capture. SCIENTIFIC REPORTS, 8(1), 9436 [10.1038/s41598-018-27819-x].
Alvarez-Cubero, M; Santiago, O; Martínez-Labarga, C; Martínez-García, B; Marrero- Díaz, R; Rubio-Roldan, A; Pérez-Gutiérrez, A; Carmona-Saez, P; Lorente, J; Martinez-Gonzalez, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/201639
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