This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.
Alvarez-Cubero, M., Santiago, O., Martínez-Labarga, C., Martínez-García, B., Marrero- Díaz, R., Rubio-Roldan, A., et al. (2018). METHODOLOGY FOR Y CHROMOSOME CAPTURE AND ANALYSIS: A complete genome sequence of Y chromosome using Flow Cytometry Capture, Laser Capture Microdissection and Magnetic Streptavidin-Bead Capture. SCIENTIFIC REPORTS, 8(1), 9436 [10.1038/s41598-018-27819-x].
METHODOLOGY FOR Y CHROMOSOME CAPTURE AND ANALYSIS: A complete genome sequence of Y chromosome using Flow Cytometry Capture, Laser Capture Microdissection and Magnetic Streptavidin-Bead Capture
Martínez-Labarga C.;
2018-01-01
Abstract
This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.File | Dimensione | Formato | |
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