We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(methacrylic acid) (PMA) capsules with tuneable mechanical properties were fabricated through layer-by-layer assembly. The Pin was quantified by correlating the cell-induced deformation with the ex situ osmotically induced deformation of the polymer capsules. Ultimately, we found that human monocyte-derived macrophage THP-1 cells exerted up to approximately 360 kPa on the capsules during internalisation.

Chen, X., Cui, J., Ping, Y., Suma, T., Cavalieri, F., Besford, Q.a., et al. (2016). Probing cell internalisation mechanics with polymer capsules. NANOSCALE, 8(39), 17096-17101 [10.1039/C6NR06657G].

Probing cell internalisation mechanics with polymer capsules

Cavalieri, Francesca;
2016-01-01

Abstract

We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(methacrylic acid) (PMA) capsules with tuneable mechanical properties were fabricated through layer-by-layer assembly. The Pin was quantified by correlating the cell-induced deformation with the ex situ osmotically induced deformation of the polymer capsules. Ultimately, we found that human monocyte-derived macrophage THP-1 cells exerted up to approximately 360 kPa on the capsules during internalisation.
2016
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02 - CHIMICA FISICA
English
Con Impact Factor ISI
Chen, X., Cui, J., Ping, Y., Suma, T., Cavalieri, F., Besford, Q.a., et al. (2016). Probing cell internalisation mechanics with polymer capsules. NANOSCALE, 8(39), 17096-17101 [10.1039/C6NR06657G].
Chen, X; Cui, J; Ping, Y; Suma, T; Cavalieri, F; Besford, Qa; Chen, G; Braunger, Ja; Caruso, F
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/201192
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 20
social impact