Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip.

Caselli, F., Reale, R., Nodargi, N., Bisegna, P. (2017). Numerical investigation of a novelwiring scheme enabling simple and accurate impedance cytometry. MICROMACHINES, 8(9), 283 [10.3390/mi8090283].

Numerical investigation of a novelwiring scheme enabling simple and accurate impedance cytometry

Caselli, F
;
Nodargi, NA;Bisegna, P
2017-01-01

Abstract

Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip.
2017
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ING-IND/34 - BIOINGEGNERIA INDUSTRIALE
Settore ICAR/08 - SCIENZA DELLE COSTRUZIONI
English
Con Impact Factor ISI
microfluidics; electrical impedance; single-cell analysis; lab-on-a-chip; finite element method; virtual laboratory
Caselli, F., Reale, R., Nodargi, N., Bisegna, P. (2017). Numerical investigation of a novelwiring scheme enabling simple and accurate impedance cytometry. MICROMACHINES, 8(9), 283 [10.3390/mi8090283].
Caselli, F; Reale, R; Nodargi, N; Bisegna, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/200784
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